Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by temporal inhibition of the anaphase-promoting complex/cyclosome

The Plant Cell
Jefri HeymanLieven De Veylder


The anaphase-promoting complex/cyclosome (APC/C) is a multisubunit ubiquitin ligase that regulates progression through the cell cycle by marking key cell division proteins for destruction. To ensure correct cell cycle progression, accurate timing of APC/C activity is important, which is obtained through its association with both activating and inhibitory subunits. However, although the APC/C is highly conserved among eukaryotes, no APC/C inhibitors are known in plants. Recently, we have identified ULTRAVIOLET-B-INSENSITIVE4 (UVI4) as a plant-specific component of the APC/C. Here, we demonstrate that UVI4 uses conserved APC/C interaction motifs to counteract the activity of the CELL CYCLE SWITCH52 A1 (CCS52A1) activator subunit, inhibiting the turnover of the A-type cyclin CYCA2;3. UVI4 is expressed in an S phase-dependent fashion, likely through the action of E2F transcription factors. Correspondingly, uvi4 mutant plants failed to accumulate CYCA2;3 during the S phase and prematurely exited the cell cycle, triggering the onset of the endocycle. We conclude that UVI4 regulates the temporal inactivation of APC/C during DNA replication, allowing CYCA2;3 to accumulate above the level required for entering mitosis, and thereby regul...Continue Reading


Mar 22, 2012·Nature Communications·Cao XuJiayang Li
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nubia EloyDirk Inzé
May 5, 2012·Plant & Cell Physiology·Shinichiro Komaki, Keiko Sugimoto
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhilong BaoJian Hua
Dec 20, 2013·Journal of Experimental Botany·Pascal GenschikMarie-Claire Criqui
Jan 23, 2014·The Plant Journal : for Cell and Molecular Biology·Elizabeth HénaffJosep M Casacuberta
Dec 17, 2014·The Arabidopsis Book·Christina M ChoiHanjo Hellmann
Jul 21, 2015·Current Opinion in Plant Biology·Ruth Eichmann, Patrick Schäfer
Feb 11, 2014·Current Opinion in Plant Biology·Christian BreuerKeiko Sugimoto
Oct 5, 2012·Molecular Plant·Jefri Heyman, Lieven De Veylder
Aug 18, 2012·Plant Signaling & Behavior·Eriko IwataMasaki Ito
Dec 3, 2014·Current Opinion in Plant Biology·Stefanie PolynLieven De Veylder
Aug 19, 2014·Frontiers in Plant Science·Shweta KalveGerrit T S Beemster
Nov 7, 2016·Current Opinion in Plant Biology·Zoltan MagyarMasaki Ito
Feb 13, 2016·PLoS Genetics·Marta CifuentesRaphael Mercier
Mar 2, 2019·Genes & Development·Rita A BatistaClaudia Köhler
May 10, 2019·The Arabidopsis Book·Pablo Bolaños-VillegasaYingxiang Wang
Feb 21, 2020·Journal of Experimental Botany·Cécile Raynaud, Maherun Nisa


Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jan 13, 1998·Current Opinion in Cell Biology·A Hershko
Jun 29, 2000·Annual Review of Biochemistry·A M Page, P Hieter
Jan 10, 2002·Developmental Cell·Ruth Grosskortenhaus, Frank Sprenger
May 4, 2002·Nature Cell Biology·Jerry Y HsuPeter K Jackson
Jun 7, 2002·Molecular Cell·Jan-Michael Peters
May 30, 2003·Molecular and Cellular Biology·Anna CastroThierry Lorca
Sep 6, 2003·Current Biology : CB·Hartmut C VodermaierJan-Michael Peters
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey J TungPeter K Jackson
Aug 30, 2005·Plant Physiology·Klaas VandepoeleLieven De Veylder
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Yoshihiro HaseAtsushi Tanaka
Jun 7, 2006·Current Biology : CB·Péter Lénárt, Jan-Michael Peters
Nov 11, 2006·Annual Review of Genetics·Dirk Inzé, Lieven De Veylder
Jan 20, 2007·Genes & Development·Yuichi J Machida, Anindya Dutta
Apr 12, 2007·Molecular & Cellular Proteomics : MCP·Jelle Van LeeneGeert De Jaeger
May 9, 2007·The Journal of Cell Biology·Barbara Di Fiore, Jonathon Pines
Oct 20, 2007·The Plant Journal : for Cell and Molecular Biology·José M Pérez-PérezBen Scheres
Mar 7, 2008·Development·Karine Narbonne-ReveauMary A Lilly
Apr 29, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Moe TateguKenichi Yoshid
Sep 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Tim LammensLieven De Veylder
Apr 18, 2009·Cell Cycle·Zakir UllahMelvin L DePamphilis
May 22, 2009·Plant Physiology·Véronique BoudolfLieven De Veylder
Jun 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marleen VanstraelenEva Kondorosi
Aug 25, 2009·Current Opinion in Plant Biology·Barbara Berckmans, Lieven De Veylder
Dec 31, 2009·The Plant Cell·Sebastian ProostKlaas Vandepoele
Mar 17, 2010·The Plant Journal : for Cell and Molecular Biology·Elodie Mathieu-RivetMichel Hernould
Aug 14, 2010·Molecular Systems Biology·Jelle Van LeeneGeert De Jaeger
Aug 25, 2010·Methods in Molecular Biology·Serena Perilli, Sabrina Sabatini
Sep 3, 2010·Current Opinion in Plant Biology·Katia MarroccoPascal Genschik
Nov 13, 2010·The Plant Journal : for Cell and Molecular Biology·Toon CoolsLieven De Veylder
Apr 5, 2011·The EMBO Journal·Janet L BurtonMark J Solomon
Sep 13, 2011·Plant Physiology·Barbara BerckmansLieven De Veylder

Related Concepts

Transformation, Genetic
Cyclin-Dependent Kinases
Cell Cycle Proteins
Structure-Activity Relationship
S Phase
Mutagenesis, Site-Directed
Cell Division
Cyclin A2
Ubiquitin-Protein Ligase Complexes
FZR2 protein, Arabidopsis

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.