Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation.

Proceedings of the National Academy of Sciences of the United States of America
Melanie KrebsKarin Schumacher

Abstract

The productivity of higher plants as a major source of food and energy is linked to their ability to buffer changes in the concentrations of essential and toxic ions. Transport across the tonoplast is energized by two proton pumps, the vacuolar H(+)-ATPase (V-ATPase) and the vacuolar H(+)-pyrophosphatase (V-PPase); however, their functional relation and relative contributions to ion storage and detoxification are unclear. We have identified an Arabidopsis mutant in which energization of vacuolar transport solely relies on the activity of the V-PPase. The vha-a2 vha-a3 double mutant, which lacks the two tonoplast-localized isoforms of the membrane-integral V-ATPase subunit VHA-a, is viable but shows day-length-dependent growth retardation. Nitrate content is reduced whereas nitrate assimilation is increased in the vha-a2 vha-a3 mutant, indicating that vacuolar nitrate storage represents a major growth-limiting factor. Zinc is an essential micronutrient that is toxic at excess concentrations and is detoxified via a vacuolar Zn(2+)/H(+)-antiport system. Accordingly, the double mutant shows reduced zinc tolerance. In the same way the vacuolar Na(+)/H(+)-antiport system is assumed to be an important component of the system that remo...Continue Reading

References

Nov 6, 1992·Science·A BertlE MacRobbie
Apr 5, 2000·Biochimica Et Biophysica Acta·M Maeshima
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Oct 23, 2003·The Journal of Biological Chemistry·Quan-Sheng QiuJian-Kang Zhu
Nov 26, 2003·Journal of Bioenergetics and Biomembranes·Christoph KlugeKarl-Josef Dietz
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Jan DettmerKarin Schumacher
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Georg StrompenUlrike Mayer
Feb 3, 2005·The Plant Journal : for Cell and Molecular Biology·Natalya Ivashikina, Rainer Hedrich
Oct 8, 2005·Science·Jisheng LiRoberto Gaxiola
Oct 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshio YamaguchiEduardo Blumwald
Mar 4, 2006·Journal of Experimental Botany·José M PardoFrancisco J Quintero
Nov 18, 2006·Journal of Experimental Botany·Enrico MartinoiaH Ekkehard Neuhaus
Dec 8, 2006·Molecular & Cellular Proteomics : MCP·Michel JaquinodJacques Bourguignon
Apr 7, 2007·FEBS Letters·Roberto A GaxiolaKarin Schumacher
May 24, 2007·Journal of Experimental Botany·Anthony J MillerDarren M Wells
Jan 8, 2008·Trends in Plant Science·Lucia DaxingerIan Furner
Apr 22, 2008·Biochimica Et Biophysica Acta·Daniel J CiprianoMichael Forgac
Sep 10, 2008·The Biochemical Journal·Cathrine Lillo
Mar 21, 2009·The Plant Journal : for Cell and Molecular Biology·Diana BeyhlRainer Hedrich

❮ Previous
Next ❯

Citations

Jan 5, 2012·The Journal of Biological Chemistry·Florian RienmüllerRainer Hedrich
Jan 5, 2011·Journal of Experimental Botany·Julie DechorgnatFrançoise Daniel-Vedele
May 1, 2010·Plant & Cell Physiology·Jenny von der Fecht-BartenbachUwe Ludewig
Sep 27, 2013·Plant & Cell Physiology·Ali FerjaniHirokazu Tsukaya
Apr 28, 2011·Plant Physiology·Sang-Jin Kim, Diane C Bassham
Feb 18, 2011·Plant Physiology·Yoichiro FukaoMasayoshi Maeshima
Jan 5, 2014·Planta·Werner E G MüllerXiaohong Wang
Mar 19, 2014·Trends in Plant Science·Ulrich DeinleinJulian I Schroeder
Apr 8, 2014·Trends in Plant Science·Cornelia EisenachEnrico Martinoia
May 29, 2014·Journal of Experimental Botany·Hsu-Liang Hsieh, Haruko Okamoto
Mar 13, 2012·Annual Review of Plant Biology·Enrico MartinoiaRéka Nagy
Jan 10, 2012·Annual Review of Plant Biology·Guohua XuAnthony J Miller
Oct 6, 2014·Current Opinion in Plant Biology·Karin Schumacher
Sep 1, 2014·Current Opinion in Plant Biology·Elias Bassil, Eduardo Blumwald
Nov 28, 2013·Rice·Kundan KumarYong-Gu Cho
Feb 27, 2015·Proceedings of the National Academy of Sciences of the United States of America·Caiji GaoLiwen Jiang
Dec 4, 2012·Plant Science : an International Journal of Experimental Plant Biology·Young Sim SonJeong Dong Bahk
Dec 27, 2011·Plant Science : an International Journal of Experimental Plant Biology·Soledad F UndurragaRoberto A Gaxiola
May 10, 2011·Current Opinion in Plant Biology·Terri A Long
Feb 12, 2011·Cell Calcium·Jon K Pittman
Aug 31, 2010·Current Opinion in Plant Biology·Karin Schumacher, Melanie Krebs
Aug 11, 2010·Journal of Plant Physiology·Katarzyna KabałaGrazyna Kłobus

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.