PMID: 2497734Apr 15, 1989Paper

Arachidonate cannot be released directly from diacyl-sn-glycero-3-phosphocholine in thrombin-stimulated platelets

The Biochemical Journal
O ColardJ Polonovski

Abstract

The origin of the arachidonate released from platelets on stimulation with thrombin was investigated by comparing the specific activities of released arachidonate and of arachidonoyl-containing phospholipids using rat platelets prelabelled with arachidonate. Quantification of the released arachidonate was determined in the presence of BW 755 C, a dual cyclo-oxygenase/lipoxygenase inhibitor, which was found not to modify the arachidonate mobilization between the platelet phospholipids. The phospholipid molecular species were analysed by h.p.l.c. of diradylglycerol benzoate derivatives of diacyl, alkylacyl and alkenylacyl classes. The labelled/unlabelled arachidonate ratio varied greatly in the phospholipids depending on whether an ether or acyl bond was present in sn-1 position of the glycerol, on the length and degree of unsaturation of this fatty chain and on the polar head group. Between 15 s and 5 min of stimulation by thrombin, the released arachidonate kept a constant specific activity which was considerably lower than the specific activity of diacyl-GPC. The specific activity of the released arachidonate was intermediate between the specific activities of the 16:0-20:4 and 18:0-20:4 species of diacyl-GPI and diacyl-GPE, a...Continue Reading

Citations

Dec 1, 1991·Lipids·E Ninio, F Joly
Jan 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M L Brown, D Deykin
Oct 17, 1998·The Journal of Biological Chemistry·B EngelmannA Hermetter
Jan 5, 1996·Biochimica Et Biophysica Acta·F H ChiltonJ D Winkler
Sep 27, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M GuichardantM Lagarde
May 8, 1992·Biochimica Et Biophysica Acta·F JolyO Colard

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