Arachidonate metabolism in cultured fibroblasts derived from normal and infarcted canine heart

Circulation Research
D R WeberS M Prescott

Abstract

Metabolites of arachidonic acid (eicosanoids) may have an important role in the healing process after myocardial infarction. We examined the ability of cardiac fibroblasts from normal and from healing infarcted ventricle to metabolize arachidonate. We induced myocardial infarction in dogs and then allowed them to recover for 1 week, at which time they were killed, and the heart was removed. Fibroblasts were harvested from normal and from the healing, infarcted areas of the left ventricle. The cells from each source were morphologically indistinguishable. There were 347 +/- 102-fold more fibroblasts cultured from the infarcted area than from the normal area. Interestingly, the infarct-derived cells had a slower doubling time (37.4 +/- 3.7 hours) than the normal cells (22.0 +/- 3.6 hours). The uptake of exogenous arachidonate and its distribution in complex lipids was the same in the cells from each area. When stimulated with the calcium ionophore, free exogenous arachidonate, bradykinin, or histamine the cells produced prostaglandin E2 and prostaglandin I2. In each case the infarct-derived cells produced from twofold to fivefold more prostaglandin than the normal cells. We also found that prostaglandin synthesis was highly depen...Continue Reading

References

Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M DayerD R Robinson
May 18, 1978·The New England Journal of Medicine·P Needleman, G Kaley
Jan 1, 1988·International Journal of Immunopharmacology·S K HuN C Rath
Oct 16, 1986·The New England Journal of Medicine·D J FitzgeraldG A FitzGerald
Dec 1, 1985·The Journal of Clinical Investigation·G A ZimmermanS M Prescott
Nov 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·C T January, B A Schottelius
Jul 20, 1983·The American Journal of Cardiology·G J DustingT J Martin
Dec 1, 1984·The Journal of Clinical Investigation·P J Whiteley, P Needleman
Nov 12, 1982·Biochimica Et Biophysica Acta·B T HymanA A Spector
Mar 1, 1983·The American Journal of Cardiology·E J BrownE Braunwald
Jul 1, 1980·Journal of Molecular and Cellular Cardiology·G G AhumadaP Needleman
Oct 1, 1982·Pflügers Archiv : European journal of physiology·G Isenberg, U Klockner
Jul 1, 1980·The American Journal of Cardiology·B I JugduttL C Becker
Nov 1, 1980·Journal of Molecular and Cellular Cardiology·H S BoltonG V Vahouny
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Jun 1, 1996·Clinical Cardiology·K T WeberL C Katwa
Jan 29, 2002·Journal of Molecular and Cellular Cardiology·Nicole ScheurenWinfried Schorb
Jan 12, 1996·The Journal of Biological Chemistry·J W AdamsS A Henderson
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R Schuette, M C LaPointe
Mar 27, 1995·Annals of the New York Academy of Sciences·K T WeberG Zhou
Apr 17, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Matthew D MartensJoseph W Gordon

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