PMID: 2504050Aug 1, 1989Paper

Arachidonic acid affects membrane ionic conductances of GH3 pituitary cells

The American Journal of Physiology
P VacherB Dufy

Abstract

Arachidonic acid (AA) stimulates prolactin release from pituitary cells, by mechanisms not yet understood. In this work, we analyzed the effects of AA on membrane ionic conductances in a clonal line of anterior pituitary cells (GH3/B6), finding time- and dose-dependent effects of AA on their membrane ionic conductances. The predominant response at concentrations between 100 nM and 10 microM was a prolongation of the action potential (AP) and an increase in the transient after-hyperpolarization potential. Voltage clamp studies showed that this was associated with a decrease in a voltage-dependent potassium current and an increase in a voltage-dependent calcium current. In some cells (30%) the effect of AP duration was less important, but spike firing was enhanced. For the highest concentrations used (1 and 10 microM) the effects described above were preceded by hyperpolarization of the cell membrane; in voltage clamp it was shown that this hyperpolarization resulted from the activation of a calcium-dependent potassium conductance suspected to be due to the release of intracellular calcium. The calcium store affected by AA was, at least in part, insensitive to vanadate and heparin. These data suggest that AA may enhance intracell...Continue Reading

Citations

Oct 11, 2014·Journal of Inherited Metabolic Disease·Cristina ColombelliValeria Tiranti
Sep 26, 2000·American Journal of Physiology. Cell Physiology·D D DensonD C Eaton
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Alison L ClarkeJoshua J Singer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.