Arachidonic acid and related methyl ester mediate protein kinase C activation in intact platelets through the arachidonate metabolism pathways

Biochemical and Biophysical Research Communications
X T FanM Castagna

Abstract

Unlike unsaturated fatty acids, which almost fully activated purified brain protein kinase C in a phosphatidylserine- and Ca2(+)-free reaction, related methyl esters were poorly active in vitro. In contrast, methyl arachidonate was revealed to be as potent as arachidonic acid in activating protein kinase C in intact platelets. Arachidonic acid-mediated activation peaked at 20 s while methyl arachidonate-mediated activation plateaued at 2 min when both lipids were added at 50 microM. At concentrations higher than 0.3 mM, all tested unsaturated fatty acids and related methyl esters were weak activators of the enzyme, with the exception of linolenic acid and methyl linolenate which evoked strong enzyme activation. However, inhibitors of arachidonate metabolism blocked both arachidonic-acid and methyl-arachidonate-induced responses. At 5 microM arachidonic acid and methyl arachidonate, protein kinase C activation was due to a cyclooxygenase product(s) whereas at 50 microM the lipoxygenase pathway was mostly involved in the reaction. Therefore, arachidonic acid and its methyl ester activate protein kinase C in platelets mainly through action of their metabolites and eicosanoid synthesis. It is suggested that such indirect protein ki...Continue Reading

References

Jul 1, 1989·Environmental Health Perspectives·C Da SilvaM Castagna
Apr 1, 1989·Current Opinion in Cell Biology·I Martelly, M Castagna
Jun 15, 1989·Biochemical and Biophysical Research Communications·A FournierA W Murray
Mar 15, 1988·European Journal of Pharmacology·R V VegesnaS T Crooke
Feb 15, 1989·The Biochemical Journal·K R Dell, D L Severson
Jan 1, 1987·Biology of the Cell·M Castagna
Jul 18, 1986·Science·Y Nishizuka
Aug 1, 1987·Carcinogenesis·M RoghaniM Castagna
Jan 1, 1986·Methods in Enzymology·T KitanoY Nishizuka
Feb 13, 1987·Biochemical and Biophysical Research Communications·M RoghaniM Castagna
Dec 30, 1986·Biochemistry·S L PelechE G Krebs
Jun 13, 1973·Nature: New Biology·L R Rohrsehneider, R K Boutwell
Feb 1, 1973·Prostaglandins·G H JouvenazD A van Dorp
Dec 15, 1971·Experientia·E Arffmann, J Glavind
Jun 15, 1984·Biochemical and Biophysical Research Communications·A CouturierM Castagna
Jan 1, 1981·Advances in Cancer Research·L Levine
Jan 1, 1959·Acta Pathologica Et Microbiologica Scandinavica·P HOLSTI

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Citations

Apr 1, 1995·Pflügers Archiv : European journal of physiology·H C Chung, N Fleming
Aug 16, 1996·Biochimica Et Biophysica Acta·F DalençonJ L Wautier
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·D E HallahanR R Weichselbaum
Jan 1, 1993·Nutrition and Cancer·M A BeluryS M Fischer
May 8, 2004·Journal of Toxicology and Environmental Health. Part a·Kelly A MericleG A Ansari
Jul 1, 1994·International Journal of Radiation Oncology, Biology, Physics·D E HallahanR R Weichselbaum
Jun 27, 2015·The Journal of Endocrinology·Maria Carolina S MendesJosé B C Carvalheira
Jun 14, 2005·Biochimica Et Biophysica Acta·Lise MadsenKarsten Kristiansen
Jun 30, 1992·Biochemistry·E J Bolen, J J Sando
Jul 18, 2000·American Journal of Physiology. Cell Physiology·K P TewariJ Cuppoletti

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