PMID: 6411051Jul 1, 1983Paper

Arachidonic acid availability and prostacyclin production by cultured human endothelial cells

Arteriosclerosis : an Official Journal of the American Heart Association, Inc
A A SpectorR L Czervionke

Abstract

When human umbilical vein endothelial cultures were grown in the presence of supplemental arachidonic acid, the cell phospholipids became enriched with arachidonic acid. Prostacyclin (PGI2) accumulated in the medium during supplementation with arachidonic acid. The capacity of these enriched cultures to produce PGI2 when subsequently incubated with either arachidonic acid or thrombin was reduced by as much as 90%, but release of arachidonic acid from the cell lipids in response to thrombin stimulation was not inhibited. Refractory cultures completely recovered the capacity to form PGI2 within 18 hours after removal of the medium containing supplemental arachidonic acid. However, recovery was prevented by cycloheximide. When enrichment with arachidonic acid was done in the presence of ibuprofen, a reversible cyclooxygenase inhibitor, PGI2 did not accumulate in the medium during supplementation, and the subsequent capacity of the cultures to produce PGI2 in response to thrombin increased by 70% to 240%. By contrast, the capacity of these supplemented cultures to convert added arachidonic acid to PGI2 did not increase. Therefore, the enhancement in thrombin-stimulated PGI2 production when the cultures are supplemented with arachid...Continue Reading

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Citations

Feb 12, 1986·Biochimica Et Biophysica Acta·C HadjiagapiouA A Spector
Nov 22, 1988·European Journal of Pharmacology·V J ToothillJ D Pearson
Jun 1, 1986·Prostaglandins·C Hadjiagapiou, A A Spector
Sep 21, 2013·Journal of Atherosclerosis and Thrombosis·Haruo Ohnishi, Yasushi Saito
Jun 1, 1985·Experimental Cell Research·A SandraA A Spector
Oct 1, 1987·Journal of Cellular Physiology·L L Stoll, A A Spector
Mar 1, 1985·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·E TremoliB B Weksler
Jan 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M L Brown, D Deykin
Aug 1, 1997·Circulation Research·N L WeintraubA A Spector
Jul 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H I SchaeferA van der Laarse
Jan 1, 1985·Acta Medica Scandinavica·G Thorgeirsson
Dec 16, 1985·Klinische Wochenschrift·U Delvos, G Müller-Berghaus
Nov 1, 1990·The American Journal of Physiology·S L HempelA A Spector
Feb 15, 1987·Archives of Biochemistry and Biophysics·C Hadjiagapiou, A A Spector

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