Arachidonic acid-induced activation of large-conductance potassium channels and membrane hyperpolarization in mouse B cells.

Pflügers Archiv : European journal of physiology
Haifeng ZhengSung Joon Kim

Abstract

Lymphocytes express voltage-gated (Kv) and Ca(2+)-activated (IKCa1) K(+) channels. Recently, we found that WEHI-231, an immature B cell line, expresses voltage-independent K(+) channels called large-conductance background K( + ) channels (LK(bg)). Arachidonic acid (AA) has attracted attention because of its potential regulatory roles in the apoptosis of immature B cells. To elucidate the functional targets of AA, we investigated the effects of AA on membrane currents, voltages, and cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) of WEHI-231 and Bal-17 cells that represent immature and mature mouse B cells, respectively. In whole-cell patch clamp, both Kv and IKCa1 were inhibited by AA. On the other hand, AA activated LK(bg) current and non-selective cationic (NSC) current in WEHI-231 while only NSC current in Bal-17. Inside-out patch clamp study showed that AA directly activates LK(bg). AA induced hyperpolarization of WEHI-231 and depolarization of Bal-17 cells, respectively. The selective functional expression of LK(bg) and their activation by AA were also confirmed in the immature B cells (B220(+)/AA4.1(+)) freshly isolated from mouse spleen. In fura-2 spectrofluorimetry, AA induced persistent increase in [Ca(2+)](c) of WEHI-2...Continue Reading

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Citations

Feb 11, 2011·American Journal of Physiology. Cell Physiology·Joo Hyun NamSung Joon Kim
Feb 22, 2017·Frontiers in Physiology·Fredrik Elinder, Sara I Liin
May 15, 2009·American Journal of Physiology. Cell Physiology·Haifeng ZhengSung Joon Kim
Oct 12, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dong Hoon ShinSung Joon Kim

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