PMID: 9436783Jan 22, 1998Paper

Arachidonic acid-induced inhibition of microglial outward-rectifying K+ current

Glia
S Visentin, G Levi

Abstract

In cultured microglial cells pro-inflammatory substances such as lipopolysaccharide and interferon-gamma induce outward-rectifying K+ channels (OR) exhibiting features of the Kv1.3 type. Here we studied the modulation of this channel by arachidonic acid (AA). Micromolar doses of AA (0.3-30 microM; ED50 1.55 microM) depressed OR currents at all the potentials tested. Such effect appeared in less than 30 s, reached the maximum in approximately 2 min, and partially reverted upon removal of AA. We then tested whether AA acted by mechanisms involving enzyme activation. The AA effect on OR remained unchanged in the presence of staurosporine (protein kinase C inhibitor), indomethacin (cyclooxygenase inhibitor), nordihydroguaiaretic acid (lipoxygenase inhibitor), and diphenylene iodonium (NADPH-oxidase inhibitor). The same effect was present in cell-free membrane patches in the outside-out configuration. In whole-cell recording AA induced the following changes in OR current: (a) decrease of OR current peak at all voltages tested; (b) increase of the activation rate and mild shift of the voltage-dependence of the activation; (c) acceleration of the inactivation rate with a concomitant appearance of a second and faster inactivation compo...Continue Reading

References

Jan 1, 1992·Glia·A R Korotzer, C W Cotman
Nov 1, 1992·Pflügers Archiv : European journal of physiology·P BéhéH Meves
Dec 7, 1992·Neuroscience Letters·W NörenbergP Illes
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 15, 1994·The Journal of Physiology·W NörenbergP Illes
Jun 1, 1994·Progress in Neurobiology·H Meves
Jan 1, 1993·Glia·H KettenmannW Walz
Apr 1, 1993·Current Opinion in Cell Biology·D Piomelli
Apr 7, 1995·The Journal of Biological Chemistry·K O Holevinsky, D J Nelson
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·E HonoréM Lazdunski
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G LeviC Agresti
Aug 1, 1995·Progress in Neurobiology·H Katsuki, S Okuda
Jan 5, 1996·Biochimica Et Biophysica Acta·F H ChiltonJ D Winkler

❮ Previous
Next ❯

Citations

Sep 17, 2002·Brain Research·Geon Young LeeJin-Ho Song
Jun 6, 2000·Trends in Neurosciences·M HernándezM Sánchez Crespo
Mar 6, 2009·American Journal of Physiology. Cell Physiology·Linda M BolandErin Casey
May 9, 2007·Phytotherapy Research : PTR·Risa SakaiKenji Watanabe
Jan 25, 2008·Pflügers Archiv : European journal of physiology·Haifeng ZhengSung Joon Kim
Oct 3, 2008·Cell Biochemistry and Biophysics·Linda M Boland, Michelle M Drzewiecki
Mar 1, 2003·The Journal of Biological Chemistry·Kirk L HamiltonDaniel C Devor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.