Arachidonic acid inhibits luteinizing hormone-stimulated progesterone production in hen granulosa cells

Biology of Reproduction
A L Johnson, J L Tilly

Abstract

Arachidonic acid has been proposed to function as a hormone-induced second messenger in a variety of mammalian endocrine tissues. The present studies were conducted to evaluate whether arachidonic acid, either added exogenously or released endogenously following treatment with physiologic (phospholipase A2) or pharmacologic (melittin) agents, influences basal and/or luteinizing hormone (LH)-induced cyclic adenosine 3',5'-monophosphate (cAMP) and progesterone production in granulosa cells from domestic hens. Phospholipase A2 (PLA2) and melittin treatments failed to alter basal concentrations of progesterone, whereas arachidonic acid had a slight stimulatory effect (only at the 50-microM dose) on progesterone levels, and no effect on cAMP. By contrast, arachidonic acid, PLA2, and melittin each inhibited LH-promoted progesterone production in a dose-dependent fashion. The inhibitory effects of arachidonic acid on the progesterone response were determined to occur both prior and subsequent to cAMP formation since cAMP levels in arachidonic acid-treated cells were attenuated after treatment with 10 ng LH or 100 microM forskolin (at 10- to 100-microM doses of arachidonic acid), and progesterone production was decreased in the presenc...Continue Reading

Citations

Feb 1, 1992·Molecular and Cellular Endocrinology·F HertelendyM Jamaluddin
Apr 19, 1996·Molecular and Cellular Endocrinology·M J MarineroM P López-Ruiz
Jan 1, 1991·Domestic Animal Endocrinology·J L Tilly, A L Johnson
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Jun 2, 2021·Scandinavian Journal of Public Health·Anne Marie Ladehoff ThomsenAndreas Ernst

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