Arachidonic acid is a physiological activator of the ryanodine receptor in pancreatic beta-cells

Cell Calcium
Orison WoolcottMd Shahidul Islam

Abstract

Pancreatic beta-cells have ryanodine receptors but little is known about their physiological regulation. Previous studies have shown that arachidonic acid releases Ca(2+) from intracellular stores in beta-cells but the identity of the channels involved in the Ca(2+) release has not been elucidated. We studied the mechanism by which arachidonic acid induces Ca(2+) concentration changes in pancreatic beta-cells. Cytosolic free Ca(2+) concentration was measured in fura-2-loaded INS-1E cells and in primary beta-cells from Wistar rats. The increase of cytosolic Ca(2+) concentration induced by arachidonic acid (150microM) was due to both Ca(2+) release from intracellular stores and influx of Ca(2+) from extracellular medium. 5,8,11,14-Eicosatetraynoic acid, a non-metabolizable analogue of arachidonic acid, mimicked the effect of arachidonic acid, indicating that arachidonic acid itself mediated Ca(2+) increase. The Ca(2+) release induced by arachidonic acid was from the endoplasmic reticulum since it was blocked by thapsigargin. 2-Aminoethyl diphenylborinate (50microM), which is known to inhibit 1,4,5-inositol-triphosphate-receptors, did not block Ca(2+) release by arachidonic acid. However, ryanodine (100microM), a blocker of ryanod...Continue Reading

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Citations

Feb 4, 2012·Zygote : the Biology of Gametes and Early Embryos·M T AjmatM I Bühler
Jan 1, 2009·The Journal of Biological Chemistry·Christian RoskerColin W Taylor
Dec 22, 2010·The Journal of Physiology·Md Shahidul Islam
May 10, 2008·Biochemical and Biophysical Research Communications·Naoya NoguchiAkira Sugawara
Jun 17, 2008·British Journal of Pharmacology·H Meves
Oct 8, 2014·Upsala Journal of Medical Sciences·Monica Sandberg, Leif Jansson
Oct 19, 2012·American Journal of Physiology. Cell Physiology·James F Markworth, David Cameron-Smith
Feb 22, 2017·Frontiers in Physiology·Fredrik Elinder, Sara I Liin

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