PMID: 2491880Jan 1, 1989Paper

Arachidonic acid is preferentially incorporated by immature megakaryocytes

The Journal of Laboratory and Clinical Medicine
P K Schick

Abstract

Megakaryocytes are bone marrow cells responsible for the synthesis of circulating platelets. Subgroups of megakaryocytes can be recognized on the bases of morphologic stage, DNA content (ploidy), and size. However, the physiologic roles and biochemical characteristics of these subgroups have not been defined. We have investigated arachidonic acid uptake in megakaryocyte subgroups. Purified guinea pig megakaryocytes were incubated with tritiated arachidonic acid, cytocentrifuged, and subjected to autoradiography. Eight hundred megakaryocytes were analyzed in four experiments by four parameters: (1) grains per cell (arachidonic acid uptake), (2) ploidy, (3) morphologic stage, and (4) size. Lipids were extracted from aliquots of megakaryocytes subjected to autoradiography and were assessed for arachidonic acid metabolism. Arachidonic acid had not been overtly metabolized nor oxygenated, and thus, radioactivity represented arachidonic acid. The capacity for arachidonic acid uptake was dependent primarily on megakaryocyte cytoplasmic maturation as judged by morphologic stage. Stage I and II megakaryocytes, immature cells, took up three times more arachidonic acid than stage III megakaryocytes and four times more than stage IV megaka...Continue Reading

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