Arachidonic acid level in cellular lipids determines the amount of prostaglandins synthesized during cell growth in tissue culture

Prostaglandins
S L HongD Deykin

Abstract

Methylcholanthrene transformed mouse fibroblast cells can be induced to synthesize prostaglandins by a short term incubation with various vasoactive agents including serum, bradykinin and thrombin or in response to mechanical detachment from the culture dish. The ability of the cells to synthesize prostaglandins upon stimulation changes during growth of the culture on the dish; the response is maximal on the first day after inoculation and decreased sharply thereafter. Feeding of the cells with fresh growth medium enhances prostaglandin production induced by all stimuli. The difference in the cell response during growth is probably not due to change of prostaglandin synthetase activity since the specific enzyme activities assayed with microsomal preparations of cells harvested from the first and third day culture are similar. However, analysis of the cellular content of arachidonic acid after saponification of the total lipid extract of cells harvested at different days of growth reveals that the level of arachidonic acid per cell during growth is parallel to the response to stimuli. It is maximal on the first day and decreases sharply on the second day and stays low on the third day. Our study suggests that the level of arachi...Continue Reading

References

Mar 15, 1977·European Journal of Biochemistry·S Hammarström
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B B WekslerE A Jaffe
Jan 1, 1977·Prostaglandins·F F SunJ C McGuire
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
May 11, 1976·Proceedings of the National Academy of Sciences of the United States of America·R W Alexander, M A Gimbrone
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S L Hong, L Levine
Aug 11, 1972·Biochimica Et Biophysica Acta·E J Christ, D A van Dorp
Jun 1, 1973·Southern Medical Journal·J Nakano, M C Koss
Apr 30, 1971·Annals of the New York Academy of Sciences·H Kunze, W Vogt

Citations

Nov 1, 1981·Prostaglandins·L Taylor, P Polgar
May 1, 1985·Prostaglandins·T BauknechtH P Zahradnik
May 1, 1980·The Journal of Clinical Investigation·A A SpectorJ B Smith
Oct 1, 1981·The Journal of Clinical Investigation·A A SpectorG L Fry
Nov 12, 1982·Biochimica Et Biophysica Acta·B T HymanA A Spector
Nov 1, 1982·Journal of Cellular Physiology·M D Rosenthal, M C Whitehurst
Jan 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M L Brown, D Deykin

Related Concepts

Metazoa
Eicosatetraenoic Acids
Bradykinin, (3-D-Pro)-Isomer
Neoplastic Cell Transformation
Fibroblasts
Methylcholanthrene
Prostaglandin-Endoperoxide Synthase
Prostaglandins E
Prostaglandins F
Alpha-Thrombin

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