PMID: 2492199Jan 23, 1989Paper

Arachidonic acid metabolism in isolated pancreatic islets. V. The enantiomeric composition of 12-hydroxy-5,8,10,14-eicosatetraenoic acid indicates synthesis by a 12-lipoxygenase rather than a monooxygenase

Biochimica Et Biophysica Acta
J TurkM L McDaniel

Abstract

Recent evidence indicates that the arachidonate metabolite 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) or its precursor may act as a second messenger in stimulus-response coupling in a variety of cells including Aplysia neurons, adrenal glomerulosa cells, and pancreatic islets. The compound 12(S)-HETE is generated from the precursor 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid (12(S)-HPETE), which is a product of the 12-lipoxygenase enzyme. Some cells have recently been found to produce the enantiomer 12(R)-HETE, apparently via a cytochrome P-450 monooxygenase, and the biologic actions of 12(R)-HETE and 12(S)-HETE differ. We have examined the stereochemistry of 12-HETE from isolated pancreatic islets both radiochemically and by a new mass spectrometric method capable of quantitating subnanogram amounts of 12-HETE stereoisomers. Endogenous 12-HETE from islets was found to be exclusively the S-isomer. D-Glucose stimulated both insulin secretion and islet accumulation of 12(S)-HETE but not of 12(R)-HETE. Pharmacologic inhibition of islet 12-HETE biosynthesis also suppressed glucose-induced insulin secretion. These findings suggest that islet 12-HETE is a product of a 12-lipoxygenase rather than of a cytochrome P-450 ...Continue Reading

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M L SchwartzmanR C Murphy
Dec 1, 1985·Biomedical Mass Spectrometry·J Y WestcottR C Murphy
Jan 1, 1986·Annual Review of Biochemistry·P NeedlemanJ B Lefkowith
Jul 2, 1987·Nature·S Bevan, J N Wood
Sep 15, 1987·Biochemical and Biophysical Research Communications·U F SchadeR Engel
Apr 14, 1986·Biochemical and Biophysical Research Communications·P M Woollard
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Oct 1, 1981·Physiological Reviews·C B Wollheim, G W Sharp
Oct 16, 1980·Biochemical and Biophysical Research Communications·W F StensonT J Sullivan
Nov 1, 1984·Prostaglandins, Leukotrienes, and Medicine·C R Pace-Asciak, J M Martin
Jul 29, 1983·Biochemical and Biophysical Research Communications·J R FalckJ Capdevila
May 15, 1980·Analytical Biochemistry·J M BoeynaemsW C Hubbard

❮ Previous
Next ❯

Citations

Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·C R Carlini, J A Guimarães
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F CatellaG A FitzGerald
May 20, 1999·The Journal of Clinical Investigation·D BleichJ L Nadler
Dec 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J Fretland, S W Djuric
Aug 14, 2003·Biochemical and Biophysical Research Communications·Konkal-Matt R PrasadJerry L Nadler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.