PMID: 2106561Mar 1, 1990Paper

Arachidonic acid metabolism varies with the state of differentiation in density gradient-separated mouse epidermal cells

The Journal of Investigative Dermatology
G S CameronS M Fischer

Abstract

Epidermal cells were isolated from adult inbred SENCAR (SSIN) mice and separated by density-gradient centrifugation. The cells were pooled into three fractions shown by previous work to differ in their state of differentiation and proliferative potential. The three fractions were examined for their capacity to metabolize exogenous 14C-arachidonic acid (AA) into prostaglandins (PG) and hydroxyeicosatetraenoic acids (HETE). Cells found in the upper two fractions, which are less dense, have less proliferative potential in vitro, and are more differentiated than cells in the lower more dense fraction, are much more active in producing PG from exogenous AA than are the more dense cells. This was observed in intact cells as well as cells disrupted by freeze-thawing following density separation. The same relationship was found for HETE production in that cytoplasmic preparations from the two fractions containing the less dense cells were much more active in the production of HETE than cytoplasmic preparations from the more dense fraction. The two upper fractions differed little from each other in the production of PG or HETE. These results indicate the presence of higher levels of active cyclooxygenase and lipoxygenases in fractions c...Continue Reading

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Citations

Nov 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ayako FutagamiNobuaki Yamanaka
Sep 20, 1996·The Journal of Biological Chemistry·C D FunkA R Brash
Sep 20, 2005·The Journal of Pharmacology and Experimental Therapeutics·Liping DuDiane S Keeney
Jul 17, 1998·Experimental Cell Research·K Müller-DeckerG Fürstenberger
Jul 1, 1996·Critical Reviews in Oncology/hematology·C Denzlinger
Mar 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G S CameronS M Fischer
Oct 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C B EvansM E Goldyne
Jan 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·F Grimminger, P Mayser
Mar 3, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·H P KimV A Ziboh
Aug 1, 1991·The Journal of Investigative Dermatology·H H Henneicke-von ZepelinE Christophers

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