PMID: 7542448Jul 1, 1995Paper

Arachidonic acid mobilization is suppressed during mitosis: role of cytosolic phospholipase A2 activation

The Biochemical Journal
R D Berlin, S F Preston

Abstract

In interphase HeLa cells, incubation with histamine or thapsigargin led to the rapid release of arachidonic acid. The release was absolutely dependent on Ca2+, consistent with the activation of an 85 kDa cytosolic phospholipase A2 (cPLA2). In metaphase-arrested HeLa cells, by contrast, the stimulation of arachidonate release by these agents was inhibited by more than 90%. The lack of arachidonic acid release by mitotic cells was at least partly expected, since histamine- or thapsigargin-induced Ca2+ influx and elevations of cytosolic free Ca2+ are known to be strongly inhibited during mitosis [Preston, Sha'afi and Berlin (1991) Cell Regul. 2, 915-925]. Indeed, incubation of interphase cells with the Ca2+ ionophore A23187 alone induced a high level of arachidonate release. However, even A23187 failed to elicit release from mitotic cells. Since the Ca(2+)-dependent release of arachidonate by many cell types is promoted by preincubation with ligands that activate receptors of the tyrosine kinase class, and tumour promoters that lead to the phosphorylation of cPLA2, we determined if the responses of mitotic HeLa cells could be modified by this 'priming' process. We first established that epidermal growth factor and phorbol 12-myris...Continue Reading

Citations

Mar 30, 1999·The Proceedings of the Nutrition Society·M J Wakelam, M M Harnett
Jul 27, 2001·Journal of Cellular Physiology·G S van RossumJ Boonstra
Oct 18, 2000·The Journal of Clinical Investigation·G J ChoukrounJ V Bonventre

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