PMID: 8606033Feb 1, 1996Paper

Arachidonic acid release: an in vitro alternative for dermal irritancy testing

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
V A DeLeoS DeSalva

Abstract

Validated in vitro alternatives are being utilized extensively for mutagenicity and ocular irritancy testing. However, validation of alternative assays for dermal irritancy is progressing more slowly. As the irritant response in human skin is mediated, at least in part, by eicosanoids derived from arachidonic acid, the effect of relatively pure anionic surfactants (AS, n=8) and surfactant-containing finished products (FP, n=25) on the release of [3H]arachidonic acid from a prelabelled murine fibroblast cell line (C3H-10T1/2 cells) in vitro was examined. Test substances were administered at various non-lethal concentrations, in triplicate, to 12- and 24-well plates containing preconfluent monolayers (80-90% confluence) of C3H-10T1/2 cells. Because it is impossible to test all concentrations of each test substance in a single assay, statistical techniques were developed to 'standardize' in vitro assay results. In each assay, radiolabel release due to a positive control was also measured, using 0.04, 0.05 and 0.06 mM concentrations of sodium dodecyl sulfate (SDS). Test substance releases were then transformed into 'SDS equivalent' responses, significantly reducing both inter- and intra-assay variability. A straight line was fitted...Continue Reading

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Citations

Oct 23, 2013·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Zouhir DjerrouYoucef Hamdi-Pacha
Sep 5, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T DeutschleH Riechelmann
Apr 28, 2009·Regulatory Toxicology and Pharmacology : RTP·Martin MacfarlaneJulia Scheel
Nov 29, 2012·Toxicology and Industrial Health·Serkan YilmazMustafa Celik
Sep 1, 1999·Alternatives to Laboratory Animals : ATLA·J van de SandtM Fartasch

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