PMID: 2492824Feb 6, 1989Paper

Arachidonic acid turnover in peritoneal macrophages is altered in endotoxin-tolerant rats

Biochimica Et Biophysica Acta
T S RogersJ A Cook

Abstract

Peritoneal macrophages from endotoxin-tolerant rats have been found to exhibit depressed metabolism of arachidonic acid (AA) to prostaglandins and thromboxane in response to endotoxin. The effect of endotoxin tolerance on AA turnover in peritoneal macrophages was investigated by measuring [14C]AA incorporation and release from membrane phospholipids. Endotoxin tolerance did not affect the amount of [14C]AA incorporated into macrophages (30 min-24 h). However, the temporal incorporation of [14C]AA into individual phospholipid pools (15 min-24 h) was altered. In endotoxin-tolerant macrophages, [14C]AA incorporation into phosphatidylcholine (PC) (2, 4, 24 h) and phosphatidylethanolamine (PE) (8 h) was increased, while the incorporation into phosphatidylserine (PS) (2-24 h) was reduced (P less than 0.005) compared to control macrophages. There was no change in [14C]AA incorporation into phosphatidylinositol (PI). Following 2 or 24 h of incorporation of [14C]AA, macrophages were incubated (3 h) with endotoxin (50 micrograms/ml) or A23187 (1 microM), and [14C]AA release was measured. Endotoxin-tolerant macrophages released decreased (P less than 0.05) amounts of [14C]AA in response to both endotoxin and the calcium ionophore A23187 c...Continue Reading

References

Feb 23, 1976·Biochimica Et Biophysica Acta·T K BillsM J Silver
Aug 1, 1987·Metabolism: Clinical and Experimental·E Rodriguez de Turco, J A Spitzer
Mar 1, 1988·The Journal of Clinical Investigation·E B Rodriguez de Turco, J A Spitzer
Jan 1, 1980·Journal of Inherited Metabolic Disease·R A ChalmersD Ogilvie
Oct 14, 1982·Biochimica Et Biophysica Acta·V G Mahadevappa, B J Holub

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Citations

Apr 13, 2000·Biochimica Et Biophysica Acta·M Szewczenko-Pawlikowski, W Kozak
Jan 1, 1990·Nutrition Research Reviews·R F Grimble
Jul 1, 1992·JPEN. Journal of Parenteral and Enteral Nutrition·B LokeshJ E Kinsella
Aug 5, 1998·The American Journal of Physiology·M Yamashita, M Yamashita

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