Arbuscular Mycorrhizal Symbiosis Leads to Differential Regulation of Drought-Responsive Genes in Tissue-Specific Root Cells of Common Bean

Frontiers in Microbiology
Gustavo H RecchiaSiu M Tsai

Abstract

Arbuscular mycorrhizal fungi (AMF) colonization in plants promotes both local and systemic changes in the gene expression profiles of the host that might be relevant for drought-stress perception and response. Drought-tolerant common bean plants (cv. BAT 477), colonized by a mixture of AMF (Glomus clarum, Acaulospora scrobiculata, and Gigaspora rosea), were exposed to a water deprivation regime of 96 h during pre-flowering. Root transcriptomes were accessed through RNA-Seq revealing a set of 9,965 transcripts with significant differential regulation in inoculated plants during a water deficit event, and 10,569 in non-inoculated. These data include 1,589 transcripts that are exclusively regulated by AMF-inoculation, and 2,313 under non-inoculation conditions. Relative gene expression analyses of nine aquaporin-related transcripts were performed in roots and leaves of plants harvested at initial stages of treatment. Significant shifts in gene expression were detected in AM water deficit-treated roots, in relation to non-inoculated, between 48 and 72 h. Leaves also showed significant mycorrhizal influence in gene expression, especially after 96 h. Root cortical cells, harboring or not arbuscules, were collected from both inoculati...Continue Reading

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Citations

Aug 29, 2019·Frontiers in Microbiology·Raffaella BalestriniIsabella Pentimone
Jun 28, 2019·Plant Direct·Eric M KramerDonald H R McClelland

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Methods Mentioned

BETA
laser-capture microdissection microscopy
Assay
RNA-Seq
electrophoresis
PCR
Light-Microscopy
Laser-Capture
Light microscopy
laser-capture microdissection
dissection

Software Mentioned

blast + package
Blast2GO
ImageJ
SAS
R
Statistical Discovery TM
Bowtie2
count
Bowtie 2
gplots

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