Arc mRNA docks precisely at the base of individual dendritic spines indicating the existence of a specialized microdomain for synapse-specific mRNA translation.

The Journal of Comparative Neurology
Joseph L Dynes, Oswald Steward

Abstract

Arc (aka Arg 3.1) is induced by neural activity and learning experience. Arc mRNA is rapidly exported into dendrites where it localizes near activated synapses. By imaging green fluorescent protein (GFP)-tagged mRNA in living neurons in culture, we show that fusion transcripts containing the Arc 30'UTR (untranslated region) localize with remarkable precision in a microdomain at the base of dendritic spines. Transcripts with the Arc 30'UTR that encode a reporter protein rather than Arc show precise localization. Localization persists in the presence of translation inhibitors, indicating that localization does not require ongoing translation. Similarly, polyribosome complexes remained stably positioned at spine bases in brain tissue treated with the translation inhibitor (puromycin) that releases ribosomes from mRNA. Single particle tracking revealed that Arc mRNA particles positioned at spine bases exhibited highly constrained submicron movements. These observations imply the existence of a microdomain at the spine base where Arc mRNA docks in association with a previously unknown mRNA-binding structural element.

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Citations

Nov 12, 2013·Cellular and Molecular Life Sciences : CMLS·María Gabriela ThomasGraciela Lidia Boccaccio
Dec 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Joseph L DynesMichael D Cahalan
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Dec 9, 2014·RNA Biology·Martyna O UrbanekWlodzimierz J Krzyzosiak
Jun 9, 2015·Trends in Cell Biology·Adina R BuxbaumHye Yoon Park
Jan 30, 2015·Frontiers in Molecular Neuroscience·Oswald StewardSarah J Van Driesche
Jan 1, 2015·Nature Reviews. Molecular Cell Biology·Adina R BuxbaumRobert H Singer
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Young J YoonRobert H Singer
Nov 8, 2016·Trends in Genetics : TIG·Rico SchieweckMichael A Kiebler
Dec 30, 2016·Molecules and Cells·Byung Hun LeeHye Yoon Park
Apr 12, 2018·Biological Chemistry·Fabienne De Graeve, Florence Besse
Jan 5, 2019·Biological Reviews of the Cambridge Philosophical Society·Alessandra DonatoMassimo A Hilliard
Apr 20, 2017·Pharmacological Reviews·Ryan T LaLumiereChrista K McIntyre
May 15, 2018·Frontiers in Behavioral Neuroscience·Francisco T GalloNoelia V Weisstaub
Dec 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Miyeon ChoiHyeon Son
May 15, 2018·Frontiers in Molecular Neuroscience·Chiara PaolantoniCorinna Giorgi
Jul 20, 2019·Nature Communications·Karl E BauerMichael A Kiebler
May 31, 2013·The Journal of Biological Chemistry·Carolina EliscovichRobert H Singer
Nov 17, 2020·Frontiers in Molecular Neuroscience·Francesco Gobbo, Antonino Cattaneo
Apr 18, 2021·The Journal of Comparative Neurology·Sofya GindinaLinnaea Ostroff

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