Archaeal community diversity and abundance changes along a natural salinity gradient in estuarine sediments

FEMS Microbiology Ecology
Gordon WebsterAndrew J Weightman

Abstract

Archaea are widespread in marine sediments, but their occurrence and relationship with natural salinity gradients in estuarine sediments is not well understood. This study investigated the abundance and diversity of Archaea in sediments at three sites [Brightlingsea (BR), Alresford (AR) and Hythe (HY)] along the Colne Estuary, using quantitative real-time PCR (qPCR) of 16S rRNA genes, DNA hybridization, Archaea 16S rRNA and mcrA gene phylogenetic analyses. Total archaeal 16S rRNA abundance in sediments were higher in the low-salinity brackish sediments from HY (2-8 × 10(7) 16S rRNA gene copies cm(-3)) than the high-salinity marine sites from BR and AR (2 × 10(4)-2 × 10(7) and 4 × 10(6)-2 × 10(7) 16S rRNA gene copies cm(-3), respectively), although as a proportion of the total prokaryotes Archaea were higher at BR than at AR or HY. Phylogenetic analysis showed that members of the 'Bathyarchaeota' (MCG), Thaumarchaeota and methanogenic Euryarchaeota were the dominant groups of Archaea. The composition of Thaumarchaeota varied with salinity, as only 'marine' group I.1a was present in marine sediments (BR). Methanogen 16S rRNA genes from low-salinity sediments at HY were dominated by acetotrophic Methanosaeta and putatively hydroge...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E F DeLong
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J L Stein, M I Simon
Dec 24, 1997·Applied and Environmental Microbiology·M A MunsonT M Embley
Oct 3, 1999·Applied and Environmental Microbiology·C VetrianiA L Reysenbach
May 12, 2001·Journal of Applied Microbiology·C AbreuA A Bordalo
Mar 28, 2002·Applied and Environmental Microbiology·Andreas TeskeHolger W Jannasch
Aug 16, 2003·Environmental Microbiology·Torsten OchsenreiterChrista Schleper
Mar 10, 2004·Applied and Environmental Microbiology·Byron C CrumpJohn E Hobbie
Apr 10, 2004·Bioinformatics·Thomas HuberPhilip Hugenholtz
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Patrick D Schloss, Jo Handelsman
Jun 3, 2005·Nature Reviews. Microbiology·Christa SchleperMelanie Jonuscheit
Jun 11, 2005·Environmental Microbiology·Natasha BanningAndrew J Weightman
Sep 24, 2005·Nature·Martin KönnekeDavid A Stahl
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christopher A FrancisBrian B Oakley
May 1, 1982·Applied and Environmental Microbiology·E SeniorD B Nedwell
Dec 1, 1982·Applied and Environmental Microbiology·R S Oremland, S Polcin
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Fumio InagakiBo B Jørgensen
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer F BiddleKai-Uwe Hinrichs
Apr 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anitra E IngallsAnn Pearson
May 16, 2006·Nature·Suzanna L BräuerStephen H Zinder
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitchell L SoginGerhard J Herndl
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cornelia WuchterJaap S Sinninghe Damsté
Aug 18, 2006·Microbiological Research·Yonghui ZengNianzhi Jiao
May 3, 2007·Environmental Microbiology·R John ParkesAndrew J Weightman
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 27, 2007·Microbial Ecology·Ricardo P VieiraOrlando B Martins
Jan 9, 2008·The ISME Journal·Andreas Teske, Ketil B Sørensen
Feb 16, 2008·Nature Reviews. Microbiology·Céline Brochier-ArmanetPatrick Forterre
Apr 2, 2008·Annals of the New York Academy of Sciences·James G Ferry, Daniel J Lessner
Apr 2, 2008·Annals of the New York Academy of Sciences·Yuchen Liu, William B Whitman

❮ Previous
Next ❯

Citations

Oct 21, 2016·Applied Microbiology and Biotechnology·Guangshan WeiZheng Gao
Mar 2, 2018·World Journal of Microbiology & Biotechnology·Limin FanPao Xu
Feb 22, 2017·Environmental Microbiology·Adrienne B NarroweChristopher S Miller
Apr 28, 2018·Biological Reviews of the Cambridge Philosophical Society·India MansourMatthias C Rillig
Nov 2, 2016·Frontiers in Microbiology·Eva C PreisnerRobert S Norman
Mar 1, 2017·The ISME Journal·Panagiota-Myrsini ChronopoulouMark Trimmer
May 26, 2017·Frontiers in Microbiology·Ashagrie GibtanHan-Seung Lee
Sep 29, 2020·Frontiers in Microbiology·Dayu ZouMeng Li
Aug 24, 2018·FEMS Microbiology Ecology·Katharina KujalaMarja Tiirola
Jan 11, 2017·Archaea : an International Microbiological Journal·Yantian MaGang Ge
Mar 9, 2021·Frontiers in Microbiology·Anna J WalleniusMike S M Jetten
Apr 25, 2020·The Science of the Total Environment·Lynn FosterJonathan R Lloyd

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

OS Strategi
UCLUST
AODC
Primer3Plus
BLASTClust
ClustalX
QIIME
MEGA4
Bellerophon
BioEdit Sequence Alignment Editor

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.