PMID: 9435219Jan 22, 1998Paper

Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes

Proceedings of the National Academy of Sciences of the United States of America
W WangGerald R Crabtree

Abstract

The SWI/SNF complex in yeast and Drosophila is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. The mechanism by which it is targeted to specific genes is poorly understood and may involve direct DNA binding and/or interactions with specific or general transcription factors. We have previously purified a mammalian complex by using antibodies against BRG1, a human homologue of SWI2/SNF2. This complex is likely functionally related to the yeast SWI/SNF complex because all five subunit identified so far (referred to as BAFs, for BRG1-associated factors) are homologues of the yeast SWI/SNF subunits. However, we now describe the cloning of the 57-kDa subunit (BAF57), which is present only in higher eukaryotes but not in yeast. BAF57 is shared by all mammalian complexes and contains a high-mobility-group (HMG) domain adjacent to a kinesin-like region. Both recombinant BAF57 and the whole complex bind four-way junction (4WJ) DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. Surprisingly, complexes with mutations in the HMG domain of BAF57 can still bind 4WJ DNA and mediate ATP-dependent nucleosome disruption. Our wor...Continue Reading

References

Oct 1, 1991·Molecular and Cellular Biology·C L MillerH Singh
Feb 24, 1989·Science·M E BianchiG Paonessa
Oct 5, 1984·Journal of Molecular Biology·M SternI Herskowitz
Aug 11, 1994·Nature·A N ImbalzanoR E Kingston
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·B R CairnsR D Kornberg
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C L PetersonM P Scott
Mar 1, 1994·Trends in Genetics : TIG·R GrosschedlJ Pagel
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P M PilS J Lippard
Feb 29, 1996·Nature·J QuinnC L Peterson
Aug 23, 1996·The Journal of Biological Chemistry·A N ImbalzanoR E Kingston
Sep 1, 1996·Genes & Development·W WangG R Crabtree
Sep 1, 1996·Genes & Development·B R CairnsR D Kornberg
Dec 27, 1996·Cell·B R CairnsR D Kornberg
Oct 7, 1997·Molecular and Cellular Biology·C Sumi-IchinoseP Chambon

❮ Previous
Next ❯

Citations

Jan 9, 2001·Journal of Cellular Physiology·M F DecristofaroB E Weissman
Apr 6, 2011·Breast Cancer Research and Treatment·M Angeles VillarongaBorja Belandia
May 9, 2002·Biochimica Et Biophysica Acta·Young Mi LeeWankee Kim
Apr 8, 2000·Current Opinion in Genetics & Development·C L Peterson, J L Workman
Jul 12, 2002·Nature·Tian H ChiGerald R Crabtree
Nov 19, 2013·Nature Cell Biology·Hsuan-Ting HuangLeonard I Zon
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dazhi LaiTian Chi
Sep 7, 2012·The Journal of Biological Chemistry·Ghia EuskirchenMichael Snyder
Apr 14, 2004·The Journal of Experimental Medicine·Parinaz AliahmadJonathan Kaye
Jul 30, 2002·The EMBO Journal·Borja BelandiaMalcolm G Parker
Dec 5, 2003·Nucleic Acids Research·Anan Ragab, Andrew Travers
Feb 26, 2004·Nucleic Acids Research·Deborah WilskerElizabeth Moran
Nov 7, 2012·Molecular and Cellular Biology·Ronald L ChandlerTerry Magnuson
Sep 26, 2000·Molecular and Cellular Biology·D W O'NeillA Bank
Jan 11, 2000·Molecular and Cellular Biology·W WangM Gorospe
Feb 25, 2000·Molecular and Cellular Biology·M VignaliJ L Workman
Apr 21, 2001·Molecular and Cellular Biology·N K BrewsterR A Singer
Apr 25, 2002·Molecular and Cellular Biology·Wengong WangMyriam Gorospe
Sep 16, 2003·Molecular and Cellular Biology·Nickolai A BarlevShelley L Berger
Dec 16, 2003·Molecular and Cellular Biology·Angus HendersonDavid John Tremethick
Mar 4, 2005·Molecular and Cellular Biology·Kevin A LinkKaren E Knudsen
Apr 12, 2005·Journal of Zhejiang University. Science. B·Chuan-Bo Chen, Tao Li
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·O PapoulasJ W Tamkun
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mohamed-Ali HakimiRamin Shiekhattar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.