Architecture and Characteristics of Bacterial Nanotubes

Developmental Cell
Gyanendra P DubeySigal Ben-Yehuda

Abstract

Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We have previously identified a mode of bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular components. Utilizing Bacillus subtilis as a model organism, we found that at low cell density, nanotubes exhibit remarkable complexity, existing as both intercellular tubes and extending tubes, with the latter frequently surrounding the cells in a "root-like" fashion. Observing nanotube formation in real time showed that these structures are formed in the course of minutes, displaying rapid movements. Utilizing a combination of super-resolution, light, and electron microscopy, we revealed that nanotubes are composed of chains of membranous segments harboring a continuous lumen. Furthermore, we discovered that a conserved calcineurin-like protein, YmdB, presents in nanotubes and is required for both nanotube production and intercellular molecular trade.

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Citations

Oct 6, 2016·Annals of the New York Academy of Sciences·Michael R Gillings
Jun 28, 2017·Journal of Medicinal Chemistry·Gabriela SeydlováDominik Rejman
Sep 16, 2018·Molecular Microbiology·Margarita KalamaraNicola R Stanley-Wall
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Aug 9, 2020·Frontiers in Molecular Biosciences·Divya Singhi, Preeti Srivastava
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Apr 24, 2020·Nature Communications·Amit K BaidyaSigal Ben-Yehuda
Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Poorna SubramanianGrant J Jensen
Oct 2, 2020·World Journal of Microbiology & Biotechnology·Juanmei ZhangGang Wang
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