Architecture and Function of Mechanosensitive Membrane Protein Lattices

Scientific Reports
Osman KahramanChristoph A Haselwandter

Abstract

Experiments have revealed that membrane proteins can form two-dimensional clusters with regular translational and orientational protein arrangements, which may allow cells to modulate protein function. However, the physical mechanisms yielding supramolecular organization and collective function of membrane proteins remain largely unknown. Here we show that bilayer-mediated elastic interactions between membrane proteins can yield regular and distinctive lattice architectures of protein clusters, and may provide a link between lattice architecture and lattice function. Using the mechanosensitive channel of large conductance (MscL) as a model system, we obtain relations between the shape of MscL and the supramolecular architecture of MscL lattices. We predict that the tetrameric and pentameric MscL symmetries observed in previous structural studies yield distinct lattice architectures of MscL clusters and that, in turn, these distinct MscL lattice architectures yield distinct lattice activation barriers. Our results suggest general physical mechanisms linking protein symmetry, the lattice architecture of membrane protein clusters, and the collective function of membrane protein lattices.

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Citations

May 10, 2019·Soft Matter·Osman Kahraman, Christoph A Haselwandter
Feb 15, 2020·Physical Review Letters·Alexandru ParaschivAnđela Šarić
May 14, 2016·Physical Review. E·Osman KahramanChristoph A Haselwandter
Nov 30, 2019·Journal of the Mechanical Behavior of Biomedical Materials·Liangliang ZhuXi Chen
Jan 30, 2021·Soft Matter·Xinyu Liao, Prashant K Purohit
Nov 21, 2020·Physical Review. E·Mingyuan Ma, Christoph A Haselwandter
May 26, 2017·Biophysical Journal·David ArgudoMichael Grabe

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Methods Mentioned

BETA
light microscopy

Software Mentioned

Gmsh
VOOM

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