Architecture of eukaryotic mRNA 3'-end processing machinery.

Science
Ana CasañalLori A Passmore

Abstract

Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined a 3.5-angstrom-resolution structure of the ~200-kilodalton polymerase module. This revealed four β propellers, in an assembly markedly similar to those of other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3'-end processing.

References

Aug 1, 1992·Molecular and Cellular Biology·J Chen, C Moore
Mar 10, 2001·Molecular and Cellular Biology·S HelmlingC L Moore
Mar 17, 2004·Analytical Chemistry·Igor V Chernushevich, Bruce A Thomson
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jan 25, 2005·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Frank SobottCarol V Robinson
Sep 3, 2005·Molecular Cell·Xiaoyuan He, Claire Moore
Apr 5, 2007·Nature Protocols·Helena Hernández, Carol V Robinson
Jun 15, 2007·Nucleic Acids Research·Takashi Ishida, Kengo Kinoshita
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Oct 20, 2007·Nature Protocols·Melissa S ClineGary D Bader
Feb 8, 2008·The EMBO Journal·Sven DanckwardtAndreas E Kulozik
Mar 5, 2008·Accounts of Chemical Research·Thomas TavernerCarol V Robinson
Apr 23, 2008·Current Protocols in Protein Science·Christoph BieniossekImre Berger
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kalyan DasGaetano T Montelione
Dec 27, 2008·Cell·Andrea ScrimaNicolas H Thomä
Dec 29, 2009·Journal of Structural Biology·Radosav S PantelicJürgen M Plitzko
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Aug 20, 2010·BMC Bioinformatics·L Steven JohnsonElon Portugaly
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Robert A NichollsGarib N Murshudov

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Citations

Jan 24, 2018·Nature Structural & Molecular Biology·Marcello ClericiMartin Jinek
Jun 9, 2018·Nature Reviews. Drug Discovery·Jean-Paul RenaudChristian Wiesmann
Dec 24, 2018·Protein Science : a Publication of the Protein Society·Milo B FaskenMurray Stewart
Jan 27, 2019·The Journal of Biological Chemistry·Murray Stewart
Sep 4, 2019·Acta Crystallographica. Section D, Structural Biology·Max E WilkinsonAna Casañal
Dec 29, 2019·Wiley Interdisciplinary Reviews. RNA·Cornelia KilchertMinna-Liisa Änkö
Nov 7, 2019·Science Translational Medicine·Christopher SwaleMohamed-Ali Hakimi
Dec 11, 2019·Nucleic Acids Research·Katarzyna BucholcZbigniew Dominski
Nov 16, 2019·Protein Science : a Publication of the Protein Society·Ana CasañalPaul Emsley
Mar 19, 2020·PLoS Genetics·Jose F VictorinoAmber L Mosley
Jun 12, 2020·Transcription·Yadong SunLiang Tong
Jun 7, 2018·Acta Crystallographica. Section D, Structural Biology·Robert A NichollsGarib N Murshudov
Jul 8, 2020·Genetics·Joshua A ArribereHeather A Hundley
Sep 25, 2018·Nucleic Acids Research·Michael LidschreiberLori A Passmore
Mar 9, 2019·Wiley Interdisciplinary Reviews. RNA·Sarah A PeckAmber L Mosley
Aug 1, 2020·Frontiers in Cell and Developmental Biology·Hayden P Huggins, Brett D Keiper
Oct 21, 2018·Nature Communications·Marc LarochelleFrançois Bachand
Sep 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Richard J WallDavid Horn
Nov 7, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Agnieszka TudekTorben Heick Jensen
Dec 23, 2019·Structure·Lyudmila Dimitrova-PaternogaJanosch Hennig
Jan 8, 2020·Developmental Cell·Matthew L LaBellaErik M Jorgensen
Sep 10, 2019·Current Opinion in Structural Biology·Ananthanarayanan KumarMartin Jinek
Feb 7, 2021·Molecular Cell·Moritz M Pfleiderer, Wojciech P Galej
Jan 6, 2021·Genes & Development·Keith Hamilton, Liang Tong
Oct 25, 2019·Genes & Diseases·Fuwen YuanQianben Wang
Dec 8, 2019·Molecular Cell·Yixiao ZhangLiang Tong
Mar 25, 2019·Methods : a Companion to Methods in Enzymology·Katlyn Hughes Burriss, Amber L Mosley
May 4, 2020·Journal of Proteomics·Elisabetta Boeri ErbaCarlo Petosa
May 1, 2021·International Journal of Molecular Sciences·Soeun HwangChang-Woo Lee
Feb 23, 2020·Journal of Chemical Information and Modeling·Michael A Cianfrocco, Elizabeth H Kellogg

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Methods Mentioned

BETA
tandem affinity purification
electrophoresis
pull-down
electron cryomicroscopy
pulldown

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