Ardipusilloside inhibits survival, invasion and metastasis of human hepatocellular carcinoma cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Lianqing LouJinhe Wang

Abstract

Ardipusilloside I is a triterpene-saponin isolated from the Traditional Chinese Medicine Ardisia pusilla A. DC. Its effects and mechanism on invasion and metastasis of liver cancer cells are unclear. The human hepatocellular carcinoma cell line HepG2 and SMMC-7721 cells were treated with different doses of Ardipusilloside I. Cellular survival, in vitro migration and invasion were evaluated. In vivo metastatic abilities of the HCC cells were detected. We further investigated expression and phosphorylation of Mek, Erk and Akt by using Western blot. MMP2 and MMP9 activities were evaluated by gelatin zymography. E-cadherin expression, Rac1 and Cdc42 activities were examined by Western blot and pull-down assay. Ardipusilloside I inhibited invasion and metastasis of HCC cells both in vitro and in vivo by reducing the protein expressions of metalloproteinase (MMP)-9 and MMP2 proteins. Ardipusilloside I activated Rac1 that enhanced E-cadherin activity and resulted in significantly less metastasis. Our findings indicate that Ardipusilloside I has the potential of inhibition of liver cancer survival, invasion and metastasis both in vitro and in vivo.

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Citations

Nov 22, 2013·BMC Complementary and Alternative Medicine·Xiao-Fang XuXiao-Juan Wang
Feb 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bing HuLing Xu
Feb 27, 2016·Cell Biochemistry and Function·Rong ZengJi-Ren Zhang
Mar 18, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bingling DaiYanmin Zhang
Aug 15, 2013·Journal of Experimental & Clinical Cancer Research : CR·Yao-Hui WangZheng-Gang Ren
Sep 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mei-Xia ZhangZhi-Xiong Long
Feb 5, 2013·Asian Pacific Journal of Tropical Medicine·Ya-Ping Li
Dec 12, 2018·Journal of Cellular Physiology·Javid Sadri NahandHossein Keyvani
May 21, 2020·Scientific Reports·Lei SongShu-Feng Liu
Jun 7, 2017·International Journal of Molecular Medicine·Hyerin ParkKi-Tae Ha

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