Abstract
Pseudomonas aeruginosa is an important opportunistic pathogen in hospital-acquired infections. As an environmental bacterium, it colonizes soil and water as well as hospital water supply and shares its natural habitat with free-living amebae. It has been shown previously that an ameba host system can be used to analyze the virulence of P. aeruginosa strains. In this study we have developed a simple amebal co-culture test with Acanthamoeba polyphaga to compare the virulence of P. aeruginosa between human and environmental isolates. Experimental study. The four state hospitals Intensive of Marseille. Eighty-one environmental strains isolated from the hospital water and 69 clinical isolates of P. aeruginosa (52 from blood cultures and 17 from bronchoalveolar lavage) were tested in the amebal co-culture test. The results show that compared with environmental isolates, the isolates of P. aeruginosa from blood cultures were significantly more virulent in the ameba assay (37% vs. 62%, p=.006). These data suggest that clinical infections due to Pseudomonas aeruginosa are due, at least in part, to bacterial virulence. Furthermore, even within the constraints of small sample size, virulence of respiratory isolates is significantly correl...Continue Reading
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