Are clinical isolates of Pseudomonas aeruginosa more virulent than hospital environmental isolates in amebal co-culture test?

Critical Care Medicine
Lukas FennerBernard La Scola

Abstract

Pseudomonas aeruginosa is an important opportunistic pathogen in hospital-acquired infections. As an environmental bacterium, it colonizes soil and water as well as hospital water supply and shares its natural habitat with free-living amebae. It has been shown previously that an ameba host system can be used to analyze the virulence of P. aeruginosa strains. In this study we have developed a simple amebal co-culture test with Acanthamoeba polyphaga to compare the virulence of P. aeruginosa between human and environmental isolates. Experimental study. The four state hospitals Intensive of Marseille. Eighty-one environmental strains isolated from the hospital water and 69 clinical isolates of P. aeruginosa (52 from blood cultures and 17 from bronchoalveolar lavage) were tested in the amebal co-culture test. The results show that compared with environmental isolates, the isolates of P. aeruginosa from blood cultures were significantly more virulent in the ameba assay (37% vs. 62%, p=.006). These data suggest that clinical infections due to Pseudomonas aeruginosa are due, at least in part, to bacterial virulence. Furthermore, even within the constraints of small sample size, virulence of respiratory isolates is significantly correl...Continue Reading

References

Apr 1, 1996·Chest·S Crouch BrewerK V Leeper
Oct 6, 1997·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·W A Rutala, D J Weber
Jan 14, 1998·Molecular Microbiology·D W Frank
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BjörkmanD I Andersson
Feb 24, 2001·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M TrautmannM Ruhnke
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·J N SteenbergenA Casadevall
Feb 15, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D L PatersonR A Bonomo
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stefan PukatzkiJohn J Mekalanos
May 11, 2002·Journal of Bacteriology·Pierre CossonThilo Köhler
Jul 3, 2002·The Journal of Experimental Medicine·Yoichi HirakataDavid P Speert
Jul 31, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Douglas B NelsonUNKNOWN Society for Healthcare Epidemiology of America
Nov 25, 2003·Nature·Thien-Fah MahGeorge A O'Toole
Jan 11, 2005·Applied and Environmental Microbiology·Maëlle MolmeretYousef Abu Kwaik

❮ Previous
Next ❯

Citations

Apr 17, 2013·Environmental Science and Pollution Research International·Helen Y BuseNicholas J Ashbolt
Nov 10, 2011·The Journal of Microbiology·Migma Dorji TamangJungmin Kim
Jul 25, 2009·Emerging Infectious Diseases·Leonilda C SantosAnna S Levin
Sep 26, 2013·Future Microbiology·Sophie Edouard, Didier Raoult
Apr 27, 2007·International Journal of Hygiene and Environmental Health·E TuméoD Talon
Jul 22, 2008·Environmental Microbiology·Vincent ThomasGilbert Greub
Jan 29, 2011·Microbiology and Immunology·Richard Stewart BradburyAlan Charles Champion
Sep 12, 2009·FEMS Microbiology Reviews·Vincent ThomasJean-Yves Maillard
May 18, 2016·British Journal of Biomedical Science·Alan Charles ChampionDavid William Edward Cochrane Reid
Sep 30, 2016·Frontiers in Microbiology·Rodolfo García-Contreras
Apr 16, 2016·Scientific Reports·Kenta WatanabeMasahisa Watarai
Jan 9, 2015·Clinical Microbiology Reviews·Jean-Christophe LagierDidier Raoult
Sep 1, 2006·Current Opinion in Critical Care·Gökhan M Mutlu, Richard G Wunderink
May 12, 2020·Frontiers in Microbiology·Benjamin RémyÉric Chabrière

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.