Are coiled-coils of dimeric kinesins unwound during their walking on microtubule?

PloS One
Zhao-Wen DuanPeng-Ye Wang

Abstract

Dimeric kinesin motor proteins such as homodimeric kinesin-1, homodimeric Ncd and heterodimeric Kar3/Vik1are composed of two head domains which are connected together by a rod-shaped, coiled-coil stalk. Despite the extensive and intensive studies on structures, kinetics, dynamics and walking mechanism of the dimers, whether their coiled-coils are unwound or not during their walking on the microtubule is still an unclear issue. Here, we try to clarify this issue by using molecular dynamics simulations. Our simulation results showed that, for Ncd, a large change in potential of mean force is required to unwind the coiled-coil by only several pairs of residues. For both Ncd and kinesin-1, the force required to initiate the coiled-coil unwinding is larger than that required for unfolding of the single [Formula: see text]-helix that forms the coiled-coil or is larger than that required to unwind the DNA duplex, which is higher than the unbinding force of the kinesin head from the microtubule in strong microtubule-binding states. Based on these results and the comparison of the sequence between the coiled-coil of Kar3/Vik1 and those of Ncd and kinesin-1, it was deduced that the coiled-coil of the Kar3/Vik1 should also be very stable....Continue Reading

References

Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·D D Hackney
Apr 1, 1996·The Journal of Cell Biology·J M Scholey
Jan 1, 1996·Annual Review of Physiology·J Howard
Apr 1, 1997·Biophysical Journal·E Evans, K Ritchie
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B Essevaz-RouletF Heslot
Jun 10, 1998·Journal of Molecular Biology·K HiroseL A Amos
Sep 2, 1998·Journal of Structural Biology·M ThormählenE Mandelkow
Oct 31, 1998·Nature·E P SablinR J Fletterick
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·E Paci, M Karplus
Jun 17, 2000·Trends in Cell Biology·B D Manning, M Snyder
Jul 6, 2000·Nature Structural Biology·R H Wade, F Kozielski
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sotaro UemuraShin'ichi Ishiwata
Jun 18, 2002·Journal of Structural Biology·Brian Tripet, Robert S Hodges
Nov 12, 2002·The EMBO Journal·Thomas G WendtAndreas Hoenger
Nov 25, 2003·Nature Cell Biology·Kuniyoshi KasedaKeiko Hirose
Dec 6, 2003·Science·Charles L AsburySteven M Block
Dec 20, 2003·Science·Ahmet YildizPaul R Selvin
Feb 5, 2004·Chembiochem : a European Journal of Chemical Biology·Jody M Mason, Katja M Arndt
Aug 9, 2005·Current Biology : CB·Lisa R SproulSusan P Gilbert
Aug 19, 2005·The EMBO Journal·Hsiao Mei Annie ChuSharyn A Endow
Dec 31, 2005·Nature·Nicholas F EndresRonald D Vale
Mar 28, 2006·Biophysical Chemistry·Ping XiePeng-Ye Wang
Dec 26, 2006·Journal of Molecular Biology·Ping XiePeng-Ye Wang
Mar 27, 2007·Cell·Günther Woehlke, Manfred Schliwa
Apr 7, 2007·Science·Maria C AlonsoRobert A Cross
Jan 11, 2008·Structure·Wonmuk HwangMartin Karplus

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Citations

Dec 3, 2016·Journal of Theoretical Biology·Si-Kao GuoPing Xie
Jan 30, 2021·Journal of Chemical Theory and Computation·Shu-Xia LiuQing Ji
Aug 13, 2021·Protein Science : a Publication of the Protein Society·Ping Xie

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