Jan 1, 1988

Are fluorescent bodies of Y-spermatozoa detectable in common with mammalan species?

Theriogenology
S OgawaH Nagashima

Abstract

An attempt was made to detect the fluorescent bodies (F-body), using Quinacrine mustard (Q-M) staining in the spermatozoa from eight mammalian species (human, bull, boar, dog, rabbit, rat, mouse, and mastomys) as well as in the cock (used as negative control). Sperm suspension, prepared after rinsing by repeated centrifugation with phosphate buffered saline (PBS), was either stained with Q-M for 24 h or treated with protease and then stained with Q-M for 60 min. The final concentration of Q-M in the mixed staining sperm suspension was 0.025 mg/ml. The examination using a reflecting fluorescent microscope revealed that the F-body found in human sperm was also present in the sperm of all the mammals but not in the cock after 24 h of staining. The enzyme-treated specimens showed higher incidences of F-bodies than specimens stained for 24 h without enzymatic digestion. These findings strongly suggest that the F-body is commonly present in the spermatozoa of many mammalian species.

  • References4
  • Citations5

References

Mentioned in this Paper

Buffers
Sperm Cell
Spermatozoa Cell Count
Phosphate Measurement
Endopeptidases
Proteolytic Enzyme
Centrifugation
Fluorescent stain
Mastomys
Digestion

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