Are other fluorescent tags used instead of ethidium bromide safer?

Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences
Soodabeh Saeidnia, Mohammad Abdollahi

Abstract

Ethidium bromide (EtBr) is a well-known fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid DNA and to alter their conformation to Z-DNA molecules that stop replication of kinetoplastid DNA leading to Trypanosoma death. Although the usual amounts used in laboratories are considered as below the level required to cause toxicity (LD50 in oral administration in rat is 1.5 g/Kg), the mentioned concentrations are high enough to involve in replication of mitochondrial DNA in some human cell lines. Regarding the points that EtBr is very stable in the environment and if degraded especially by use of bleaches that result in formation of mutagenic compounds, there is a big concern about its use. Although application of EtBr is going to be restricted and replaced with other tags such as SYBR® products, the safety of the new substituted compounds are still in doubt and except a few data, there is no essential evidence available to confirm that they are safer than EtBr. Further investigations are recommended to compare their relative biosafety hazards.

References

May 1, 1987·Analytical Biochemistry·G Lunn, E B Sansone
Jun 1, 1994·Trends in Biochemical Sciences·P N Hengen
Sep 22, 2010·Nucleic Acids Research·Amanda C GentryNeil Osheroff
Dec 29, 2010·PLoS Pathogens·Arnab Roy ChowdhuryPaul T Englund
Mar 8, 2013·Experimental Neurology·Nicholas J KuypersScott R Whittemore

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Citations

Aug 13, 2015·Nucleic Acids Research·Seonghyun LeeDavid C Schwartz
May 10, 2020·The Analyst·Xuelin JinKyubong Jo
Feb 1, 2021·Ageing Research Reviews·Alexander S SokolovAlexey A Moskalev
Apr 9, 2021·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Haruna TaharaMasaaki Kurata

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Methods Mentioned

BETA
electrophoresis

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