Are phloem sieve tubes leaky conduits supported by numerous aquaporins?

American Journal of Botany
Ryan C StanfieldJoan Laur

Abstract

Aquaporin membrane water channels have been previously identified in the phloem of angiosperms, but currently their cellular characterization is lacking, especially in tree species. Pinpointing the cellular location will help generate new hypotheses of how membrane water exchange facilitates sugar transport in plants. We studied histological sections of balsam poplar (Populus balsamifera L.) in leaf, petiole, and stem organs. Immuno-labeling techniques were used to characterize the distribution of PIP1 and PIP2 subfamilies of aquaporins along the phloem pathway. Confocal and super resolution microscopy (3D-SIM) was used to identify the localization of aquaporins at the cellular level. Sieve tubes of the leaf lamina, petiole, and stem were labeled with antibodies directed at PIP1s and PIP2s. While PIP2s were mostly observed in the plasma membrane, PIP1s showed both an internal membrane and plasma membrane labeling pattern. The specificity and consistency of PIP2 labeling in sieve element plasma membranes points to high water exchange rates between sieve tubes and adjacent cells. The PIP1s may relocate between internal membranes and the plasma membrane to facilitate dynamic changes in membrane permeability of sieve elements in re...Continue Reading

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Citations

Mar 16, 2017·Journal of Integrative Plant Biology·Martin D VenturasUwe G Hacke
Dec 21, 2017·F1000Research·Johannes Liesche, John Patrick
Apr 12, 2019·Journal of Experimental Botany·Aart J E van Bel, Rita Musetti
Feb 16, 2018·Current Opinion in Plant Biology·Sanna Sevanto
Apr 29, 2018·Current Opinion in Plant Biology·Fulton E RockwellN Michele Holbrook

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