PMID: 9431682Jan 1, 1997Paper

Are the characteristics of betanidin glucosyltransferases from cell-suspension cultures of Dorotheanthus bellidiformis indicative of their phylogenetic relationship with flavonoid glucosyltransferases?

Planta
Thomas VogtDieter Strack

Abstract

Uridine 5'-diphosphoglucose:betanidin 5-O- and 6-O-glucosyltransferases (5-GT and 6-GT; EC 2.4.1) catalyze the regiospecific formation of betanin (betanidin 5-O-beta-glucoside) and gomphrenin I (betanidin 6-O-beta-glucoside), respectively. Both enzymes were purified to near homogeneity from cell-suspension cultures of Dorotheanthus bellidiformis, the 5-GT by classical chromatographic techniques and the 6-GT by affinity dye-ligand chromatography using UDP-glucose as eluent. Data obtained with highly purified enzymes indicate that 5-GT and 6-GT catalyze the indiscriminate transfer of glucose from UDP-glucose to hydroxyl groups of betanidin, flavonols, anthocyanidins and flavones, but discriminate between individual hydroxyl groups of the respective acceptor compounds. The 5-GT catalyzes the transfer of glucose to the C-4' hydroxyl group of quercetin as its best substrate, and the 6-GT to the C-3 hydroxyl group of cyanidin as its best substrate. Both enzymes also catalyze the formation of the respective 7-O-glucosides, but to a minor extent. Although the enzymes were not isolated to homogeneity, chromatographic, electrophoretic and kinetic properties proved that the respective enzyme activities were based on the presence of single...Continue Reading

Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·Bong-Gyu KimJoong-Hoon Ahn
Feb 16, 2006·Plant Cell Reports·Jae Hyung KoJoong-Hoon Ahn
Apr 18, 2007·Plant Molecular Biology·Luzia V ModoloRichard A Dixon
Mar 23, 2001·Phytochemistry·N KobayashiW Schliemann
Mar 7, 2003·Phytochemistry·Dieter StrackWillibald Schliemann
Oct 22, 2003·Phytochemistry·Catherine M KramerGerson Graser
Nov 17, 2001·Phytochemistry·Willibald SchliemannDieter Strack
Aug 15, 2000·Plant Science : an International Journal of Experimental Plant Biology·G TaguchiM Okazaki
Sep 28, 1999·Trends in Plant Science·P J Facchini
Feb 20, 2002·The Plant Journal : for Cell and Molecular Biology·U von RadM Frey
May 20, 2008·Journal of Experimental Botany·Markus GriesserWilfried Schwab
May 4, 2006·Annual Review of Plant Biology·Dianna BowlesFabián E Vaistij
Jun 24, 2006·Bioscience, Biotechnology, and Biochemistry·Jeong Ho KimJoong-Hoon Ahn
Feb 5, 2013·Phytochemistry·Vasko Veljanovski, C Peter Constabel
Mar 17, 2007·Journal of Plant Physiology·Jae Hyung KoJoong-Hoon Ahn
Jun 24, 2015·Phytochemistry·Mohammad Imtiyaj Khan, P Giridhar
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Yoshikazu TanakaAkemi Ohmiya
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Setsuko ShimadaMasaaki Sakuta
Jun 30, 2011·The New Phytologist·Samuel F BrockingtonDouglas E Soltis
Dec 1, 2007·Journal of Biochemistry and Molecular Biology·Byoung Seok HongJoong-Hoon Ahn
Aug 31, 2013·Plant Science : an International Journal of Experimental Plant Biology·Shibendu Sekhar DasSatyahari Dey
Apr 28, 2006·FEMS Microbiology Letters·Jae Hyung KoAhn Joong-Hoon
Jan 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xingyuan XiBaolong Liu
Apr 10, 1999·Plant Physiology·W SchliemannD Strack
Jun 29, 2021·Frontiers in Plant Science·Ramona GrütznerSylvestre Marillonnet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.