PMID: 10799463May 9, 2000

Are the enzyme immunoassays for antibodies to pneumococcal capsular polysaccharides serotype specific?

Clinical and Diagnostic Laboratory Immunology
A SoininenH Käyhty

Abstract

The specificity of antibody binding to pneumococcal capsular polysaccharides (Pnc PSs) measured by enzyme immunoassay (EIA) was studied by inhibition of antibody binding by homologous and heterologous PSs. We found extensive cross-reactivity of antibody binding to type 6B, 19F, and 23F PSs but not to type 14 PS, even after treatment with cell wall PS (CPS). The cross-reactive antibody was highly prevalent in sera of infants and adults with naturally acquired antibody, but not in sera of infants and adults immunized with pneumococcal vaccines. However, a type 11A antibody response was seen after vaccination with heterologous PSs. Monoclonal antibodies prepared against a type 6B PS-tetanus toxoid conjugate recognized also other than the specific type of PS in the EIA, implying the possible existence of a cross-reactive epitope. Remarkable differences in specificity among type 6B PS preparations from different manufacturers were found. Moreover, different lots of type 11A PS from the same manufacturer showed differences in specificity. The results suggest that some Pnc PS preparations may contain cross-reactive epitopes or impurities, other than CPS, that are common to many types of Pnc PS. The specificity of antibodies, especiall...Continue Reading

References

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Feb 1, 1991·American Journal of Diseases of Children·H KäyhtyL Saarinen
Jun 1, 1984·Chest·P B Davis, P A di Sant'Agnese
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Citations

Dec 26, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H Käyhty, H Ahman
Apr 15, 2003·Clinical Microbiology Reviews·Andrew S ArtzDan L Longo
Jun 21, 2007·American Journal of Clinical Pathology·Jerry W PickeringHarry R Hill
May 15, 2002·The Pediatric Infectious Disease Journal·A SoininenH Käyhty
Sep 11, 2003·Clinical and Diagnostic Laboratory Immunology·Raymond E BiaginiDavid N Weissman
Apr 10, 2002·American Journal of Clinical Pathology·Jerry W PickeringHarry R Hill
Aug 7, 2003·Expert Review of Vaccines·P Helena Mäkelä, H Käyhty

Related Concepts

Monoclonal Antibodies
Antibody Specificity
Antigens, Bacterial
Bacterial Vaccines
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Polyglobin
Pneumococcal Infections
Streptococcus pneumoniae
Seroprevalence

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