ARF6 GTPase protects the post-mitotic midbody from 14-3-3-mediated disintegration.

The EMBO Journal
Nimesh JosephMasanori Mishima

Abstract

In cytokinesis, there is a lengthy interval between cleavage furrow ingression and abscission, during which the midbody microtubule bundle provides both structural support for a narrow intercellular bridge and a platform that orchestrates the biochemical preparations for abscission. It is currently unclear how the midbody structure is stably maintained during this period. Here, we report a novel role for the ADP-ribosylation factor 6 (ARF6) GTPase in the post-mitotic stabilisation of midbody. Centralspindlin kinesin-6/RhoGAP complex, a midbody component critical for both the formation and function of the midbody, assembles in a sharp band at the centre of the structure in a manner antagonised by 14-3-3 protein. We show that ARF6 competes with 14-3-3 for binding to centralspindlin such that midbodies formed by centralspindlin mutants that can bind 14-3-3 but not ARF6 frequently collapse before abscission. These data indicate a novel mechanism for the regulation of midbody dynamics in which ARF6 protects the compacted centralspindlin assembly from dissipation by 14-3-3.

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Citations

Sep 20, 2012·Journal of Cell Science·Kian-Yong LeeMasanori Mishima
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Jul 9, 2014·Journal of Cell Science·Elizabeth Faris CrowellArnaud Echard
Oct 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Han PanZhucheng Chen

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