Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice

PloS One
Yue-Hong ShenShao-Xing Zhu

Abstract

CAR is a transmembrane protein that is expressed in various epithelial and endothelial cells. CAR mediates adenoviral infection, as well as adenovirus-mediated oncolysis of AxdAdB-3, an E1A/E1B double-restricted oncolytic adenovirus, in prostate cancer cells. This study further assessed the therapeutic efficacy of AxdAdB-3 with Arg-Gly-Asp (RGD)-fiber modification (AxdAdB3-F/RGD), which enables integrin-dependent infection, in prostate cancer. Susceptibility of prostate cancer cells LNCaP, PC3, and DU145 to adenovirus infection was associated with CAR expression. All of the prostate cancer cell lines expressed integrin αvβ3 and αvβ5. AxdAdB-3 was more cytopathic in CAR-positive prostate cancer cells than in CAR-negative cells, whereas AxdAdB3-F/RGD caused potent oncolysis in both CAR-positive and CAR-negative prostate cancer cells. In contrast, AxdAdB3-F/RGD was not cytopathic against normal prostate epithelial cells, RWPE-1. Intratumoral injection of AxdAdB3-F/RGD into CAR-negative prostate cancer cell xenografts in nude mice inhibited tumor growth. The current study demonstrates that E1A/E1B double-restricted oncolytic adenovirus with an RGD-fiber modification enhances infection efficiency and anti-tumor activity in CAR-defic...Continue Reading

References

Jan 1, 1993·The Prostate·A G CarrollE P Gelmann
Dec 19, 2002·Cancer Gene Therapy·Hironari DehariHirofumi Hamada
Mar 12, 2004·Oncogene·Candelaria Gomez-ManzanoJuan Fueyo
Oct 8, 2004·The New England Journal of Medicine·Ian F TannockUNKNOWN TAX 327 Investigators
May 17, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mariko WakayamaKazunari K Yokoyama
May 27, 2011·The New England Journal of Medicine·Johann S de BonoUNKNOWN COU-AA-301 Investigators
Jun 2, 2011·International Journal of Cancer. Journal International Du Cancer·Sari PesonenAkseli Hemminki
Nov 15, 2011·The Journal of Urology·Patrick C Walsh
Apr 25, 2012·Prostate Cancer·Teresa Maria Santos AmaralLuis Costa
Aug 17, 2012·The New England Journal of Medicine·Howard I ScherUNKNOWN AFFIRM Investigators
Oct 13, 2014·Annals of Diagnostic Pathology·Katharina HeßChristoph Röcken
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
electrophoresis
xenograft
xenografts
fluorescence-activated cell sorting

Software Mentioned

Image J
SPSS

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.