ARG1 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight.

Astrobiology
Agata K ZupanskaAnna-Lisa Paul

Abstract

Scientific access to spaceflight and especially the International Space Station has revealed that physiological adaptation to spaceflight is accompanied or enabled by changes in gene expression that significantly alter the transcriptome of cells in spaceflight. A wide range of experiments have shown that plant physiological adaptation to spaceflight involves gene expression changes that alter cell wall and other metabolisms. However, while transcriptome profiling aptly illuminates changes in gene expression that accompany spaceflight adaptation, mutation analysis is required to illuminate key elements required for that adaptation. Here we report how transcriptome profiling was used to gain insight into the spaceflight adaptation role of Altered response to gravity 1 (Arg1), a gene known to affect gravity responses in plants on Earth. The study compared expression profiles of cultured lines of Arabidopsis thaliana derived from wild-type (WT) cultivar Col-0 to profiles from a knock-out line deficient in the gene encoding ARG1 (ARG1 KO), both on the ground and in space. The cell lines were launched on SpaceX CRS-2 as part of the Cellular Expression Logic (CEL) experiment of the BRIC-17 spaceflight mission. The cultured cell lines ...Continue Reading

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Citations

Jan 20, 2019·International Journal of Molecular Sciences·Agata K ZupanskaAnna-Lisa Paul
Mar 21, 2020·Frontiers in Plant Science·Brandon CalifarRobert J Ferl
Mar 11, 2020·Frontiers in Plant Science·Lucius Wilhelminus Franciscus MuthertGiovanna Aronne

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Datasets Mentioned

BETA
GSE81442
GDS744

Methods Mentioned

BETA
ISS
PCR
chemical treatments

Software Mentioned

Bioconductor
WOLF PSORT
arrayQualityMetrics
AmiGO
Primer Express
AgriGO
adjust
NOT
agriGO PAGE
Affymetrix MAS

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