ARG1 Gene Polymorphisms and Their Association in Individuals with Essential Hypertension: A Case-Control Study

DNA and Cell Biology
Syed Fawad Ali ShahSabir Hussain

Abstract

The purpose of this study is to investigate the association of variant alleles (rs2781666 and rs2781667) at ARG1 to be involved in the generation of essential hypertension (EH) phenotypes in human subjects. The ARG1 noncoding polymorphisms (rs2781666; Chr6:131572419-G/T and rs2781667; Chr6:131573754-C/T) were investigated in 570 subjects, including 285 individuals diagnosed with EH. Determination of serum arginase activity and concentrations of nitric oxide catabolites were detected by the colorimetric enzymatic assay. Genetic typing of the noncoding polymorphisms, in ARG1, was performed using PCR and restriction digestion strategy. A significant increase in arginase activity was observed in individuals exhibiting EH phenotypes, compared with controls (p < 0.0001). Arginase showed negative correlation with serum nitrite and nitrate (r = -0.446 and r = -0.6075, respectively). A significant difference to be claimed in the distribution of SNPotypes, in rs2781666 and rs2781667, between cases and controls (p = 0.0086 and p = 0.0232; respectively). Interestingly, variant allele T, at both loci, is tightly linked to the disease phenotypes compared to the wild-type allele (p = 0.002; and p = 0.007, respectively). To our knowledge, this...Continue Reading

References

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Citations

Dec 12, 2018·Genetic Testing and Molecular Biomarkers·Syed Fawad Ali ShahSabir Hussain
Mar 7, 2021·Nitric Oxide : Biology and Chemistry·Caroline C Pinto-SouzaValeria C Sandrim
Nov 28, 2021·Journal of Clinical Medicine·Monika Buraczynska, Izabela Zakrocka

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Methods Mentioned

BETA
ELISA
PCR
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad Instat

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