PMID: 8945918Nov 1, 1996Paper

Arginase activity in endothelial cells: inhibition by NG-hydroxy-L-arginine during high-output NO production

The American Journal of Physiology
G M BugaL J Ignarro

Abstract

Rat aortic endothelial cells were found to contain both constitutive and lipopolysaccharide (LPS)-inducible arginase activity. Studies were performed to determine whether induction of nitric oxide synthase (NOS) by LPS and cytokines is accompanied by sufficient arginase induction to render arginine concentrations rate limiting for high-output NO production. Unactivated cells contained abundant arginase activity accompanied by continuous urea formation. LPS induced the formation of both inducible NOS (iNOS) and arginase, and this was accompanied by increased production of NO, citrulline, and urea. Immunoprecipitation experiments revealed the constitutive presence of arginase-I in both unactivated and LPS-activated cells and arginase-II induction by LPS. Arginase-I and iNOS were verified by reverse transcriptase-polymerase chain reaction. Induction of large amounts of iNOS by LPS plus several cytokines resulted in large quantities of NO, citrulline, and NG-hydroxy-L-arginine (NOHA), but urea production was markedly diminished. Decreased urea production was attributed to increased formation of NOHA, the precursor to NO and citrulline and a potent inhibitor of arginase-I activity with an inhibitory constant of 10-12 microM. Inhibit...Continue Reading

Citations

Jan 25, 2000·American Journal of Physiology. Cell Physiology·A A OgonowskiR W Caldwell
Jun 11, 2002·Annual Review of Nutrition·Sidney M Morris
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia
Jul 12, 2003·American Journal of Physiology. Heart and Circulatory Physiology·David A WinkNazareno Paolocci
Sep 15, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiling XuSerpil C Erzurum
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Jon M FukutoDavid A Wink
Jun 13, 2015·Oxidative Medicine and Cellular Longevity·Luiza A RabeloMarília O F Goulart
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C ZhangL Kuo
Feb 24, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Louis G ChicoineLeif D Nelin
Jan 16, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Scott HorowitzParvaneh Rafiee
Sep 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Masatoshi ImamuraHiroshi Azuma
Nov 20, 2016·Medicinal Research Reviews·Marc PudloCorine Girard-Thernier
Jul 5, 2005·American Journal of Respiratory Cell and Molecular Biology·Leif D NelinYusen Liu
Dec 20, 2000·American Journal of Physiology. Endocrinology and Metabolism·H LiG Wu
Dec 14, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Fruzsina K JohnsonWilliam Durante
Nov 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D Kepka-LenhartS M Morris
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michael G JanechDavid W Ploth
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·L D NelinL G Chicoine
Feb 12, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tetsu KawaguchiRaymond C Koehler
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Seiichi MochizukiFumihiko Kajiya
Sep 13, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kate P StanleyLeif D Nelin
Oct 18, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Travis W HeinLih Kuo
Apr 20, 2007·American Journal of Physiology. Cell Physiology·Leif D NelinYusen Liu
Dec 4, 2019·Intensive Care Medicine Experimental·Simon Lambden
Jul 23, 2004·Physiological Reviews·Fabio L M RicciardoloGert Folkerts
May 27, 2021·Cellular and Molecular Life Sciences : CMLS·Adrià-Arnau Martí I Líndez, Walter Reith
Feb 16, 2010·Archives of Biochemistry and Biophysics·Luigi Di CostanzoDavid W Christianson
Mar 23, 2010·Nitric Oxide : Biology and Chemistry·Simon Daff
Nov 28, 2021·Journal of Clinical Medicine·Monika Buraczynska, Izabela Zakrocka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.