Arginase activity is modulated by IL-4 and HOArg in nephritic glomeruli and mesangial cells

The American Journal of Physiology
S N WaddingtonV Cattell

Abstract

Arginase shares a common substrate, L-arginine, with nitric oxide synthase (NOS). Both enzymes are active at inflammatory sites. To understand regulation of arginase and its relationship to nitric oxide (NO) production, we studied effects of NG-hydroxy-L-arginine (HOArg) and interleukin-4 (IL-4) on urea and NO2- synthesis by glomeruli during rat immune glomerulonephritis and compared these with macrophages and glomerular mesangial cells (MC). In nephritic glomeruli, elicited macrophages, and MC stimulated with IL-1 and adenosine 3',5'-cyclic monophosphate agonists, increased arginase and induced NOS activity was found. Urea production was inhibited by HOArg and increased by IL-4. NO inhibition [NG-monomethyl-L-arginine (L-NMMA)] increased arginase activity in nephritic glomeruli and macrophages but not MC. NO2- synthesis was inhibited by L-NMMA and IL-4. It was increased with HOArg under conditions of NO inhibition. In contrast, in normal glomeruli and basal MC, where there was no induced NO synthesis, IL-4 had no effect on arginase activity, whereas HOArg consistently reduced it in glomeruli only. Type II arginase (Arg II) mRNA was detected in normal glomeruli; nephritic glomeruli expressed both Arg I and Arg II mRNAs. This is...Continue Reading

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Citations

May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C ZhangL Kuo
Feb 24, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Louis G ChicoineLeif D Nelin
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia
Sep 13, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kate P StanleyLeif D Nelin
Apr 20, 2007·American Journal of Physiology. Cell Physiology·Leif D NelinYusen Liu
Apr 8, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Motoyoshi EndoTomomi Gotoh
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·L D NelinL G Chicoine

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