Arginine and glutamine availability and macrophage functions in the obese insulin-resistant Zucker rat

Journal of Cellular Physiology
Marie-Céline BlancJean-Pascal De Bandt

Abstract

Increased susceptibility to infections in obese patients may be related to decreased availability of arginine and glutamine, which may affect immune cell functions. Our aim was to evaluate the in vitro effects of these amino acids on the function of macrophages from obese insulin-resistant Zucker rats. Macrophages, isolated from male Zucker obese or lean rats by peritoneal lavage, were incubated in Dulbecco's modified Eagle medium (DMEM) without arginine or glutamine. Arginine or glutamine was added to the medium at increasing final concentrations (0, 0.25, 0.5, 1 or 2 mM). After stimulation by lipopolysaccharide (LPS) from E. coli (40 microg/ml), productions of tumour necrosis factor alpha (TNFalpha) and of nitric oxide (NO) were measured after 3 or 48 h incubation, respectively. NO production, lower in macrophages from obese rats, decreased in macrophages from lean rats (0 mM: 2,423 +/- 1,174 vs. 2 mM: 198 +/- 31 microM/mg protein/24 h; P < 0.05), but not in those from obese rats, when glutamine was added. TNFalpha production, lower in macrophages from obese rats, was inversely correlated with glutamine concentration. In the presence of arginine, NO production was constantly higher in macrophages from obese rats. It peaked at...Continue Reading

References

Aug 1, 1978·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·G HolmP Björntorp
May 15, 1992·The Biochemical Journal·R G BogleG E Mann
Apr 1, 1991·Metabolism: Clinical and Experimental·M JeevanandamW R Schiller
Jun 9, 1988·The New England Journal of Medicine·H R MichieD W Wilmore
Mar 1, 1982·The American Journal of Medicine·E J RayfieldH Smith
Jul 1, 1996·Diabetes·M StumvollJ Gerich
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·T GainsfordD J Hilton
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Q YangA M Diehl
Feb 6, 1998·Journal of Applied Physiology·S MoriguchiE Shimizu
Jun 17, 1998·Research in Immunology·B SinhaS Endres
Feb 10, 1999·The American Journal of Physiology·F Y LeeA M Diehl
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·J P De Bandt, L A Cynober
Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·D T Efron, A Barbul
Apr 20, 2000·JPEN. Journal of Parenteral and Enteral Nutrition·X L CuiS Ogoshi
Feb 13, 2001·Cytokine·M CoëffierP Déchelotte
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L ConnellyA J Hobbs
Feb 1, 1994·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·P C Calder

❮ Previous
Next ❯

Citations

Feb 16, 2011·Vaccine·Prathibha BandaruGiridharan Nappanveettil
Apr 16, 2010·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Djamel HamaniChristophe Moinard
Dec 21, 2011·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Charlotte BreuillardSylviane Darquy
Nov 14, 2014·The British Journal of Nutrition·Charlotte BreuillardJean-Pascal De Bandt
Jun 23, 2006·The Lancet Infectious Diseases·Matthew E Falagas, Maria Kompoti
Jul 21, 2005·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·H-J AnH-M Kim
May 13, 2021·Clinical Oral Investigations·Nur BalciMuhittin A Serdar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Related Papers

Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition
Erich Roth
Metabolism: Clinical and Experimental
C Wallace, D Keast
© 2021 Meta ULC. All rights reserved