PMID: 9422358Jan 9, 1998Paper

Arginine and lysine aminopeptidase activities in chromaffin granules of bovine adrenal medulla: relevance to prohormone processing

Journal of Neurochemistry
Sukkid YasothornsrikulVivian Hook

Abstract

Conversion of prohormones and neuropeptide precursors to smaller, biologically active peptides requires specific proteolytic processing at paired basic residues, which generates intermediate peptides with NH2 and COOH termini extended with Lys or Arg residues. These basic residues are then removed by aminopeptidase and carboxypeptidase activities, respectively. Among the proteases involved in prohormone processing, the basic residue aminopeptidase activity has not been well studied. This report demonstrates arginine and lysine aminopeptidase activities detected with Arg-methylcoumarinamide (Arg-MCA) and Lys-MCA substrates in neurosecretory vesicles of bovine adrenal medulla [chromaffin granules (CG)], which contain endoproteolytic processing enzymes co-localized with [Met]enkephalin and other neuropeptides. These arginine and lysine aminopeptidase activities showed many similarities and some differences. Both arginine and lysine aminopeptidase activities were stimulated by the reducing agent beta-mercaptoethanol (beta-ME) and inhibited by p-hydroxymercuribenzoate, suggesting involvement of reduced cysteinyl residues. The arginine aminopeptidase activity was stimulated by NaCl (150 mM), but the lysine aminopeptidase activity was...Continue Reading

Citations

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S R Davis-KaplanJ Kaplan
Feb 14, 2006·Neurochemical Research·Maria Hui, Koon-Sea Hui
Mar 7, 2012·The Journal of Biological Chemistry·Lydiane FunkelsteinVivian Hook
Nov 19, 2015·The Journal of Endocrinology·Rafaela Fadoni AlpontiPaulo Flavio Silveira
Jul 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sukkid YasothornsrikulVivian Hook
May 4, 2011·Journal of Molecular Neuroscience : MN·Hisatsugu KoshimizuY Peng Loh
Oct 5, 2010·The Journal of Biological Chemistry·Christina C SpevakMatthew S Sachs
Nov 20, 2002·Annals of the New York Academy of Sciences·Vivian Hook, Marie-Hélène Metz-Boutigue
Jan 11, 2008·Annual Review of Pharmacology and Toxicology·Vivian HookShin-Rong Hwang
Jun 13, 2006·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Eric W DicksonGerene M Denning
Nov 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Elaine Gasparello-Clemente, Paulo Flávio Silveira
Dec 17, 2009·Regulatory Peptides·Hisatsugu KoshimizuY Peng Loh

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