Abstract
Since arginine deiminase (ADI; EC 3.5.3.6) inhibits cell proliferation by arresting cells in the G1 phase, we tested its synergistic effect on cell death induced by dexamethasone (DEX), which also induces apoptosis by G1 cell cycle arrest. ADI inhibited cell proliferation and induced apoptosis in human leukemic CEM cells in a dose-dependent manner. Simultaneous treatment with ADI and DEX showed synergistic effects on DNA fragmentation and LDH release. In addition, ADI exerted its anti-proliferative activity against DEX-resistant CEM cells. ADI suppressed expression of c-myc, a potential key regulator of cell proliferation and apoptosis, and increased expression of p27Kip1 cyclin-dependent kinase inhibitor. These results suggest that ADI efficiently increases the anti-cancer effect of DEX on human leukemic CEM cells through G1 cell cycle arrest involving downregulation of c-myc and upregulation of p27Kip1.
References
Feb 1, 1979·Journal of Cellular Physiology·J M HarmonE B Thompson
May 11, 1992·FEBS Letters·M Rabinovitz
May 8, 1992·International Journal of Cancer. Journal International Du Cancer·H TakakuK Miyazaki
Jan 1, 1997·Experimental Parasitology·L A KnodlerM R Edwards
Feb 1, 1997·International Journal of Hematology·Y KomadaE Azuma
May 1, 1997·The Annals of Pharmacotherapy·L M Holle
Jun 25, 1998·Infection and Immunity·B A DegnanJ A Goodacre
Nov 10, 1998·The Biochemical Journal·G Wu, S M Morris
Dec 8, 1998·Pediatric Hematology and Oncology·S SahuS H Advani
Jul 16, 1999·Biochemical and Biophysical Research Communications·H GongL Schweigerer
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·E B ThompsonB H Johnson
Dec 10, 1999·Immunopharmacology and Immunotoxicology·H O PaeH T Chung
Feb 2, 2000·Leukemia Research·S H ChoiB G Chun
Jun 10, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·D N WheatleyS Smith
Aug 23, 2000·British Journal of Cancer·L ScottD N Wheatley
Mar 12, 2002·Advances in Cancer Research·Sara K OsterLinda Z Penn
Apr 2, 2002·Neoplasia : an International Journal for Oncology Research·Aaron L MillerE Brad Thompson
Jun 18, 2002·Cancer Letters·Karin BeloussowWei Chiang Shen
Mar 6, 2003·Cancer Letters·Li-Jiuan ShenWei-Chiang Shen
Jul 17, 2003·International Journal of Cancer. Journal International Du Cancer·Hong GongLothar Schweigerer
Aug 28, 2003·British Journal of Cancer·I-S ParkB-H Min
Citations
Aug 23, 2005·Cancer Letters·Denys N WheatleyElaine Campbell
Jul 25, 2009·The British Journal of Nutrition·Jong-Eun KimHyong Joo Lee
Jul 6, 2012·Cell Death & Disease·B DelageP W Szlosarek
Oct 30, 2010·Pigment Cell & Melanoma Research·Tin-Lun LamYun-Chung Leung
Jan 8, 2013·Clinical Pharmacology : Advances and Applications·Jung-Ki YoonKevin B Kim
Jun 6, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Lihua LiHaifeng Song
Sep 23, 2014·BMC Cancer·Jiangbo LiuQingyong Ma
Jul 21, 2014·Enzyme and Microbial Technology·Keum-Young AhnJeewon Lee
Jun 22, 2006·Expert Opinion on Investigational Drugs·Lynn Feun, Niramol Savaraj
Jan 14, 2009·Cancer Letters·T L LamY C Leung
Jan 16, 2007·Protein Expression and Purification·Jong-Eun KimHyong Joo Lee
Nov 11, 2006·International Journal of Cancer. Journal International Du Cancer·Cheol-Yong YoonBon-Hong Min
Apr 29, 2015·Cancer Letters·Fuming QiuMeihua Sui
Apr 26, 2016·Oncogene·M D PatilD Fukumura
Oct 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paolo A AsciertoTheodore F Logan
Dec 3, 2011·British Journal of Cancer·M P KellyG Ritter
Apr 16, 2019·Expert Review of Molecular Diagnostics·Giuseppe LucarelliMichele Battaglia
Dec 24, 2018·Apoptosis : an International Journal on Programmed Cell Death·Lucy E MétayerGuy C Brown
Jan 11, 2007·Cancer Research·Paul Ning-Man ChengYun-Chung Leung
Jul 25, 2021·Cancers·Chia-Lin ChenHsing-Jien Kung
Jul 25, 2021·International Journal of Molecular Sciences·Chunjing WuNiramol Savaraj
Jul 27, 2021·Cancer Chemotherapy and Pharmacology·Neha Kumari, Saurabh Bansal