Arginine depletion by arginine deiminase does not affect whole protein metabolism or muscle fractional protein synthesis rate in mice

PloS One
Juan C Marini, Inka C Didelija

Abstract

Due to the absolute need for arginine that certain cancer cells have, arginine depletion is a therapy in clinical trials to treat several types of cancers. Arginine is an amino acids utilized not only as a precursor for other important molecules, but also for protein synthesis. Because arginine depletion can potentially exacerbate the progressive loss of body weight, and especially lean body mass, in cancer patients we determined the effect of arginine depletion by pegylated arginine deiminase (ADI-PEG 20) on whole body protein synthesis and fractional protein synthesis rate in multiple tissues of mice. ADI-PEG 20 successfully depleted circulating arginine (<1 μmol/L), and increased citrulline concentration more than tenfold. Body weight and body composition, however, were not affected by ADI-PEG 20. Despite the depletion of arginine, whole body protein synthesis and breakdown were maintained in the ADI-PEG 20 treated mice. The fractional protein synthesis rate of muscle was also not affected by arginine depletion. Most tissues (liver, kidney, spleen, heart, lungs, stomach, small and large intestine, pancreas) were able to maintain their fractional protein synthesis rate; however, the fractional protein synthesis rate of brain,...Continue Reading

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Citations

Nov 13, 2015·Current Opinion in Clinical Nutrition and Metabolic Care·Juan C Marini
Jul 31, 2018·Expert Opinion on Drug Metabolism & Toxicology·Shenglei YanJingqing Zhang
Dec 24, 2019·The Journal of Nutrition·Juan C Marini
Jul 2, 2019·Experimental and Therapeutic Medicine·Junjun NiLijun Wu
Dec 1, 2020·PloS One·Kristina M CrossNicolaas E P Deutz
Nov 15, 2020·The Journal of Nutrition·Mahmoud A MohammadJuan C Marini
Jan 12, 2021·American Journal of Physiology. Endocrinology and Metabolism·Mahmoud A MohammadJuan C Marini
May 4, 2021·Journal of Pharmacological and Toxicological Methods·Sven JäckelMichael W Schmitt
May 16, 2020·Cancer Cell·Mylène Tajan, Karen H Vousden
Aug 20, 2021·Journal of Pharmacological and Toxicological Methods·Sven JäckelMichael W Schmitt

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