Arginine facilitates inactivation of enveloped viruses

Journal of Pharmaceutical Sciences
Hisashi YamasakiTsutomu Arakawa

Abstract

Virus inactivation is a key step for the purification of pharmaceutical proteins derived from recombinant mammalian expression systems and conventionally done using low pH-treatment, which is often harmful to the proteins to be purified. This is particularly true for antibodies, because immunoglobulin proteins undergo conformational changes at acidic pH. We have been developing mild elution solvents using arginine for Protein-A chromatography to minimize the low pH-induced damages on the antibodies. Here we have tested the aqueous solutions containing arginine or butyroyl-arginine at or above pH 4.0 for their effects on virus inactivation, since these solvents are effective above pH 4.0 in elution of bound antibodies from Protein-A columns. When the virus was incubated on ice, 0.1 M sodium citrate was totally ineffective above pH 4.0, but aqueous solutions containing arginine above 0.35 M or butyroyl-arginine above 0.28 M showed extensive virus killing at or even above pH 4.0.

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Citations

Feb 9, 2012·Advances in Virology·Hisashi YamasakiA Hajime Koyama
Jun 4, 2010·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tsutomu Arakawa
Dec 1, 2009·Vaccine·Satoshi OhtakeVu Truong-Le
Oct 11, 2008·International Journal of Pharmaceutics·Hirotoshi UtsunomiyaTsutomu Arakawa
Jul 12, 2008·International Journal of Pharmaceutics·Yukiko KatsuyamaTsutomu Arakawa
Jan 22, 2009·Biotechnology Journal·Tsutomu ArakawaA Hajime Koyama
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May 27, 2014·Biochimica Et Biophysica Acta·Tsutomu ArakawaDaisuke Ejima
Jul 8, 2015·Antiviral Chemistry & Chemotherapy·Evgeny Vlad Butorov
May 2, 2018·Japanese Journal of Infectious Diseases·Hisataka GodaA Hajime Koyama
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Satoshi OhtakeA Hajime Koyama
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