Arginine increases development of in vitro-produced porcine embryos and affects the protein arginine methyltransferase-dimethylarginine dimethylaminohydrolase-nitric oxide axis

Reproduction, Fertility, and Development
Bethany K RedelRandall S Prather

Abstract

Culture systems promote development at rates lower than the in vivo environment. Here, we evaluated the embryo's transcriptome to determine what the embryo needs during development. A previous mRNA sequencing endeavour found upregulation of solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (SLC7A1), an arginine transporter, in in vitro- compared with in vivo-cultured embryos. In the present study, we added different concentrations of arginine to our culture medium to meet the needs of the porcine embryo. Increasing arginine from 0.12 to 1.69mM improved the number of embryos that developed to the blastocyst stage. These blastocysts also had more total nuclei compared with controls and, specifically, more trophectoderm nuclei. Embryos cultured in 1.69mM arginine had lower SLC7A1 levels and a higher abundance of messages involved with glycolysis (hexokinase 1, hexokinase 2 and glutamic pyruvate transaminase (alanine aminotransferase) 2) and decreased expression of genes involved with blocking the tricarboxylic acid cycle (pyruvate dehydrogenase kinase, isozyme 1) and the pentose phosphate pathway (transaldolase 1). Expression of the protein arginine methyltransferase (PRMT) genes PRMT1, PRMT3 and PRMT5...Continue Reading

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Citations

Jul 28, 2016·PloS One·Jason R HerrickRebecca L Krisher
Feb 20, 2019·Molecular Reproduction and Development·Bethany R MordhorstRandall S Prather
Oct 17, 2019·Biology of Reproduction·Joao G N MoraesThomas E Spencer
Feb 13, 2018·Experimental and Therapeutic Medicine·Yong Wang, Liyan Jin
Jul 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ye YuanR Michael Roberts
Oct 14, 2018·Transgenic Research·Kristin M WhitworthRandall S Prather
Sep 4, 2019·Molecular Reproduction and Development·Caroline G LucasTiago Collares
Jun 3, 2021·Molecular Reproduction and Development·Paula R ChenRandall S Prather
Jun 10, 2021·Scientific Reports·Francisco A García-VázquezMª José Izquierdo-Rico
Nov 25, 2021·Science Translational Medicine·Joan K LunneyChaohui Dai

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Methods Mentioned

BETA
PCR
chromosomal aberrations
antisense oligonucleotide

Software Mentioned

SAS
ImageJ

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