Arginine-induced insulin secretion in endoplasmic reticulum

Biochemical and Biophysical Research Communications
Makoto UmedaTakeshi Imai

Abstract

Arginine, a semi-essential amino acid, is known as one of the most strongest insulin secretagogues in a glucose-dependent manner, but major mechanism is unknown. Arginine induced insulin secretion in mice as well as β cell line, NIT-1, in which more than 90% of intracellular insulin is prionsulin without arginine cultivation. Arginine administration reduced prionsulin amount in 30 s, then insulin is secreted from NIT1 cells. These data indicated that the target factor(s) for arginine-induced insulin secretion located in endoplasmic reticulum (ER). We established the screening system for identifying the arginine mimetics. Brazilian propolis, not Chinese propolis, induced insulin secretion. To identify target factor(s) of arginine induced insulin secretion, our previous study was that nanobeads technology facilitated us to purify chemical-target factors. This time we chose the other way, proinsulin associating factor purification and arginine-immobilized agarose. Three proinsulin associating factors and 5 arginine interacting factors were identified. Among theses factors, Calnexin (CNX) was the only one factor, which belonged to both groups, suggesting that CNX might play a key role in arginine-induced insulin secretion in ER.

References

Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ImaiD Metzger
Feb 16, 2002·The EMBO Journal·Tiziana AnelliRoberto Sitia
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takeshi ImaiDaniel Metzger
Dec 4, 2004·Molecular Pharmacology·Petrine WellendorphHans Bräuner-Osborne
Apr 25, 2006·Biochemical and Biophysical Research Communications·Takashi KudoMasatoshi Takeda
Mar 1, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Flávia Aparecida ResendeEliana Aparecida Varanda
Sep 17, 2013·British Journal of Pharmacology·C ClemmensenH Bräuner-Osborne
Dec 17, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tatsuya UebiTakeshi Imai
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Daiki NakatsuMasayuki Murata
Jul 15, 2015·Clinical and Experimental Gastroenterology·Makoto UmedaTakeshi Imai

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Citations

Mar 24, 2016·Biochemical and Biophysical Research Communications·Yong-Wei NieDong-Jun Liu
Oct 13, 2017·Biology of Reproduction·Haijun GaoChandra Yallampalli
Dec 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hiroshi Kitamura
Jan 20, 2021·The Journal of Clinical Investigation·Ming LiuPeter Arvan
Mar 6, 2021·Nutrición hospitalaria·María Jesús Lisbona GonzálezJavier Díaz Castro
May 19, 2019·Biochemical and Biophysical Research Communications·Yoji TsugawaTakeshi Imai
May 20, 2020·Biochemical and Biophysical Research Communications·Jaeyong ChoTakeshi Imai
Apr 13, 2021·Molecular and Cellular Biochemistry·Monica Losada-Barragán

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