Arginine kinase: a common feature for management of energy reserves in African and American flagellated trypanosomatids

The Journal of Eukaryotic Microbiology
Claudio Alejandro PereiraM M Flawiá

Abstract

This work reports the characterization of an arginine kinase in the unicellular parasitic flagellate Trypanosoma brucei, the etiological agent of human sleeping sickness and Nagana in livestock. The arginine kinase activity, detected in the soluble fraction obtained from procyclic forms, had a specific activity similar to that observed in Trypanosoma cruzi, about 0.2 micromol min(-1) mg(-1). Western blot analysis of T. brucei extracts revealed two bands of 40 and 45 kDa. The putative gene sequence of this enzyme had an open reading frame for a 356-amino acid polypeptide, one less than the equivalent enzyme of T. cruzi. The deduced amino acid sequence has an 82% identity with the arginine kinase of T. cruzi, and highest amino acid identities of both trypanosomatids sequences, about 70%, were with arginine kinases from the phylum Arthropoda. In addition, the amino acid sequence possesses the five arginine residues critical for interaction with ATP as well as two glutamic acids and one cysteine required for arginine binding. The finding in trypanosomatids of a new phosphagen biosynthetic pathway, which is not present in mammalian host tissues, suggests this enzyme as a possible target for chemotherapy.

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Citations

Dec 21, 2010·Amino Acids·Ehud InbarClaudio A Pereira
Oct 1, 2013·International Journal of Biological Macromolecules·Oluyomi Stephen Adeyemi, Chris George Whiteley
Jul 26, 2011·Journal of Helminthology·M NagatakiT Agatsuma
Jul 28, 2009·Parasitology·Mariana R MirandaClaudio A Pereira
Jun 23, 2010·Experimental Parasitology·Simone A KikuchiOctavio Fernandes
Apr 28, 2007·Experimental Parasitology·Gaspar E CanepaClaudio A Pereira
Mar 25, 2006·Molecular Microbiology·Pninit Shaked-MishanDoris Rentsch
Sep 11, 2004·Biochemical and Biophysical Research Communications·León A BouvierClaudio A Pereira
May 24, 2011·Enzyme Research·Claudio A PereiraMariana R Miranda
Oct 27, 2011·Molecular and Biochemical Parasitology·Darren J CreekMichael P Barrett
Jun 1, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gaspar E CanepaClaudio A Pereira
Mar 27, 2010·FEMS Microbiology Letters·Carolina CarrilloClaudio A Pereira
Oct 27, 2015·The Journal of Eukaryotic Microbiology·Nubia C MancholaAriel M Silber
Jun 2, 2016·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Gen ZhangPei-Yuan Qian
Nov 5, 2003·FEBS Letters·Claudio A PereiraMirtha M Flawiá
May 15, 2003·The Journal of Eukaryotic Microbiology·Claudio A PereiraMirtha M Flawiá
Jun 8, 2019·Expert Opinion on Drug Delivery·Daniele Ribeiro de AraújoEneida de Paula
Jun 6, 2009·Journal of Biomolecular Structure & Dynamics·Q ShengS-J Yao
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oluyomi Stephen AdeyemiGaber El-Saber Batiha
Nov 25, 2020·FEMS Microbiology Letters·Edward A Valera-VeraClaudio A Pereira
Nov 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mauro SerricchioPeter Bütikofer
Mar 5, 2008·Journal of Proteome Research·Yannick PauchetSusanne Preiss
Apr 26, 2021·Cellular Microbiology·Estefanía Calvo-ÁlvarezBrice Rotureau
Jul 16, 2005·Science·Matthew BerrimanNajib M El-Sayed

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Datasets Mentioned

BETA
AQ658136
AQ656418
AF206293

Methods Mentioned

BETA
phosphotransferase
PCR

Software Mentioned

BLAST
BLOCKS

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.