PMID: 18187867Jan 12, 2008Paper

Arginine-modified DNA aptamers that show enantioselective recognition of the dicarboxylic acid moiety of glutamic acid

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Kazuomi OhsawaHiroaki Sawai

Abstract

We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and their binding affinities for the target were evaluated by binding assays using affinity gels. Three modified DNA molecules having higher affinity were sequenced and we determined their affinity and specificity for the target by surface plasmon resonance (SPR) measurements. The SPR studies indicated that two of these three aptamers distinguished the dicarboxylic acid moiety of the D-isomer from that of the L-isomer; however, the third aptamer did not show enantioselectivity.

References

Jan 17, 1995·Biochemistry·D E Huizenga, J W Szostak
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R F MacayaJ Feigon
Aug 15, 1996·Nucleic Acids Research·A Ortiz, E Ritter
May 1, 1997·Nature Biotechnology·R R Breaker
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y LiD Schubert
Jun 1, 1997·Current Opinion in Chemical Biology·R R Breaker
Jun 11, 1999·Current Opinion in Structural Biology·Y Li, R R Breaker
Sep 20, 2000·Accounts of Chemical Research·M FamulokM Blind
Feb 1, 1995·Journal of the American Chemical Society·C T Lauhon, J W Szostak
Sep 7, 2001·Journal of the American Chemical Society·S W Santoro Barbas CF 3rd
May 23, 2002·Cellular and Molecular Life Sciences : CMLS·G M Emilsson, R R Breaker
Aug 22, 2002·Journal of the American Chemical Society·Leonard LermerDavid M Perrin
Aug 31, 2002·Nucleic Acids Research·Heike A Held, Steven A Benner
Jan 29, 2003·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Sandeep VermaMichael Famulok
Jul 10, 2003·Chembiochem : a European Journal of Chemical Biology·Mohammad Mehedi MasudHiroaki Sawai
Sep 27, 2003·Nucleic Acids Research. Supplement·Masayasu KuwaharaHiroaki Sawai
Oct 14, 2003·Bioorganic & Medicinal Chemistry Letters·Masayasu KuwaharaHiroaki Sawai
Feb 26, 2004·Bioorganic & Medicinal Chemistry·Mohammad Mehedi MasudHiroaki Sawai
Apr 1, 2004·Journal of the American Chemical Society·Jonathan P MayDavid M Perrin
Jun 24, 2004·Angewandte Chemie·Stefan Jäger, Michael Famulok
Oct 8, 2004·Journal of the American Chemical Society·Richard TingDavid M Perrin
Jun 25, 2005·Organic & Biomolecular Chemistry·Tsutomu OhbayashiHiroaki Sawai
Oct 27, 2005·Journal of the American Chemical Society·Stefan JägerMichael Famulok
Sep 5, 2006·Journal of Molecular Biology·Jungsan Sohn, Johannes Rudolph
Feb 1, 2007·Journal of the American Chemical Society·Atsushi ShojiHiroaki Sawai
Nov 5, 2001·Angewandte Chemie·Oliver ThumMichael Famulok

❮ Previous
Next ❯

Citations

Oct 1, 2008·Histochemistry and Cell Biology·Paul Debbage, Werner Jaschke
Dec 16, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mei HuaYunhui Yang
Nov 15, 2012·Journal of Nucleic Acids·Maureen McKeague, Maria C Derosa
Oct 25, 2012·Journal of Nucleic Acids·Yuuya Kasahara, Masayasu Kuwahara
Jul 28, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erkai LiuDavid M Perrin
Sep 22, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stella Diafa, Marcel Hollenstein
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Sep 18, 2013·Artificial DNA, PNA & XNA·Masayasu Kuwahara, Satoshi Obika
Feb 22, 2017·Chemical Communications : Chem Comm·I S MacPhersonI J Krauss
Jul 11, 2009·Chembiochem : a European Journal of Chemical Biology·Marcel HollensteinDavid M Perrin
Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fadwa OdehSaid I Ismail
Jan 18, 2020·Nucleic Acids Research·Sougata Dey, Jonathan T Sczepanski
Nov 13, 2018·Nucleic Acids Research·Lars VerdonckJosé C Martins
Oct 12, 2020·Bioorganic & Medicinal Chemistry Letters·Kunihiko MorihiroSatoshi Obika
Feb 19, 2021·Journal of Agricultural and Food Chemistry·Emily MastronardiMaria C DeRosa
Jan 26, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shin-Woong KimTakanori Uzawa
Jun 6, 2013·Journal of the American Chemical Society·Yuri ImaizumiNaoki Sugimoto
Oct 7, 2017·Journal of Agricultural and Food Chemistry·Emily MastronardiMaria C DeRosa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.