Arginine mutations within a transmembrane domain of Tar, an Escherichia coli aspartate receptor, can drive homodimer dissociation and heterodimer association in vivo

The Biochemical Journal
Neta Sal-Man, Yechiel Shai

Abstract

The interactions between the TM (transmembrane) domains of many membrane proteins are important for their proper functioning. Mutations of residues into positively charged ones within TM domains were reported to be involved in many genetic diseases, possibly because these mutations affect the self- and/or hetero-assembly of the corresponding proteins. To our knowledge, despite significant progress in understanding the role of various amino acids in TM-TM interactions in vivo, the direct effect of positively charged residues on these interactions has not been studied. To address this issue, we employed the N-terminal TM domain of the aspartate receptor (Tar-1) as a dimerization model system. We expressed within the ToxR TM assembly system several Tar-1 constructs that dimerize via polar- or non-polar amino acid motifs, and mutated these by replacement with a single arginine residue. Our results have revealed that a mutation in each of the motifs significantly reduced the ability of the TMs to dimerize. Furthermore, a Tar-1 construct that contained two arginine residues was unable to correctly integrate itself into the membrane. Nevertheless, an exogenous synthetic Tar-1 peptide containing these two arginine residues was able to ...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A A Pakula, M I Simon
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K Oosawa, M Simon
Mar 1, 1994·Nature Structural Biology·M A LemmonD M Engelman
May 6, 1998·Protein Science : a Publication of the Protein Society·B Brosig, D Langosch
Jun 23, 1998·The Journal of Biological Chemistry·G B McGaugheyA K Rappé
Oct 16, 1999·Science·M Gerstein, C Chothia
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·X Han, L K Tamm
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·H GratkowskiW F DeGrado
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·F X ZhouD M Engelman
Jun 13, 2001·The Journal of Biological Chemistry·D Gerber, Y Shai
Dec 14, 2001·The Journal of Biological Chemistry·Jeannine M MendrolaMark A Lemmon
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ehud Gazit
Jan 24, 2004·Protein Science : a Publication of the Protein Society·Weiming RuanDieter Langosch
Feb 27, 2004·The Journal of Biological Chemistry·Doron GerberYechiel Shai

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Citations

Mar 10, 2007·Journal of Microbiological Methods·Eric LindnerDieter Langosch
Sep 14, 2011·Biochimica Et Biophysica Acta·Edwin LiKalina Hristova
Dec 14, 2011·Biochimica Et Biophysica Acta·Avner FinkYechiel Shai
Mar 3, 2021·Journal of Clinical Immunology·Fang YuKhaled Machaca

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