PMID: 2505450Sep 1, 1989Paper

Arginine or lysine in position 333 of ERA and CVS glycoprotein is necessary for rabies virulence in adult mice

Virology
C TuffereauA Flamand

Abstract

Fixed rabies virus strains (ERA and CVS) produce a fatal paralytic disease in mice after intracerebral or intramuscular injection. Some antigenic mutants of both CVS and ERA viruses with a substitution in position 333 of the glycoprotein (arginine is replaced either by a glutamine, a glycine, or an isoleucine) are totally avirulent for adult mice whatever the dose and the route of inoculation. Here we report an exhaustive investigation of the effect of amino acid 333 on viral virulence. New antigenic mutants were isolated from either CVS, CVS derivatives, or SADBern having arginine in position 333 encoded by CGG, AGG, CGU, or AGA respectively. This study shows that when arginine is replaced by either a leucine, an isoleucine, a methionine, a cysteine, or a serine, the antigenic mutant is also totally avirulent. But when arginine is replaced by a lysine it is still pathogenic although the LD50 by the intracerebral route is higher. Furthermore 41 independent virulent revertants were isolated from four avirulent mutants (with a glycine, a glutamine, a methionine, or a serine in position 333 of the glycoprotein). Thirty-nine regained an arginine at position 333 and 2 had a lysine. From this analysis it appears that the presence of ...Continue Reading

References

May 1, 1982·Journal of Virological Methods·F BussereauD Pese-Part

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Citations

Aug 25, 2009·Archives of Virology·Nobuyuki MochizukiTakeo Sakai
Feb 8, 2011·Archives of Virology·Takashi MatsumotoAkira Nishizono
Apr 12, 2013·Archives of Virology·Jiao WenqiangLiu Jixing
Jun 1, 1990·Veterinary Microbiology·H Le BloisA Flamand
Nov 1, 1993·Trends in Microbiology·A FlamandC Tuffereau
May 12, 2005·Virus Research·Stefan Finke, Karl-Klaus Conzelmann
Dec 31, 2002·Journal of Virological Methods·Ken ichi InoueKinjiro Morimoto
Apr 9, 1999·Vaccine·P P Pastoret, B Brochier
Jun 1, 1991·Current Opinion in Biotechnology·P P Pastoret, B Brochier
Nov 7, 1998·The Veterinary Journal·P P Pastoret, B Brochier
Jan 16, 2009·Epidemiology and Infection·W MarkotterL H Nel
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J L FuR P Roos
Dec 17, 2008·Journal of Virology·Adriane MarschalekKarl-Klaus Conzelmann
Jul 24, 2003·Journal of Neurology, Neurosurgery, and Psychiatry·P G E Kennedy
Nov 10, 2013·PLoS Neglected Tropical Diseases·Joe KgaladiLouis H Nel
Mar 25, 2008·Uirusu·Naoto Ito, Makoto Sugiyama
Jan 1, 2012·Uirusu·Akira Nishizono, Kentaro Yamada

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